DETERMINATION OF THE CAPACITY RATIO AND CONCENTRATION-TIME FUNCTION FOR STEPWISE ELUTION WITH BINARY MOBILE PHASE WITH HELP OF LIQUID-CHROMATOGRAPHY DATA OBTAINED FROM ISOCRATIC ELUTION

被引：7

作者：

JARONIEC, M ^{[1
]}

BOROWKO, M ^{[1
]}

NARKIEWICZ, J ^{[1
]}

PATRYKIEJEW, A ^{[1
]}

GOLKIEWICZ, W ^{[1
]}

机构：

[1] MED ACAD LUBLIN,INST BAS CHEM SCI,DEPT INORGAN & ANALYT CHEM,PL-20081 LUBLIN,POLAND

来源：

CHROMATOGRAPHIA | 1979年 / 12卷 / 01期

关键词：

D O I：

10.1007/BF02271596

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for the calculation of the capacity ratio and concentration-time function, basing on the theory of gradient elution chromatography, is examined by using high-speed liquid chromatography data. It is shown that the average capacity ratio and mobile-phase gradient may be easily calculated with help of experimental data obtained from isocratic elution. Using literature data, the capacity ratios predicted for stepwise elution by means of this method, have been compared with experimental data. © 1979 Friedr. Vieweg & Sohn Verlagsgesellschaft mbH.

引用

页码：29 / 32

页数：4

共 9 条

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[9] GRADIENT ELUTION IN LIQUID-CHROMATOGRAPHY .1. INFLUENCE OF COMPOSITION OF MOBILE PHASE ON CAPACITY RATIO (RETENTION VOLUME, BAND WIDTH, AND RESOLUTION) IN ISOCRATIC ELUTION - THEORETICAL CONSIDERATIONS [J].

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