CAMP REGULATION OF PHENOBARBITAL-MEDIATED INDUCTION OF DELTA-AMINOLEVULINATE SYNTHASE MESSENGER-RNA IN HEPATOCYTES FROM NORMAL AND EXPERIMENTAL DIABETIC RATS

被引：8

作者：

VARONE, CL ^{[1
]}

CANEPA, ET ^{[1
]}

LLAMBIAS, EBC ^{[1
]}

GRINSTEIN, M ^{[1
]}

机构：

[1] UNIV BUENOS AIRES, FAC CIENCIAS EXACTAS & NAT, DEPT QUIM BIOL, PORFIRINAS LAB, RA-1428 BUENOS AIRES, DF, ARGENTINA

来源：

BIOCHEMISTRY AND CELL BIOLOGY | 1994年 / 72卷 / 9-10期

关键词：

DELTA-AMINOLEVULINATE SYNTHASE MESSENGER-RNA; PHENOBARBITAL; CAMP; DIABETIC RAT HEPATOCYTES;

D O I：

10.1139/o94-051

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We examined the mechanism underlying the effect of cAMP on delta-aminolevulinate synthase mRNA biosynthesis in isolated hepatocytes from normal and experimental diabetic rats. We have demonstrated that the potentiation by dibutyryl cAMP of the phenobarbital-mediated induction of delta-aminolevulinate synthase enzyme activity, observed in our previously reported studies, reflects an increased amount of its mRNA. The inducing effect exerted by phenobarbital on the biosynthesis of delta-aminolevulinate synthase mRNA in diabetic hepatocytes is greater than that observed in normal cells. This enhanced response to the increased level of endogenous cAMP in diabetic hepatocytes is apparently sufficient for a maximum activation of the cAMP-dependent protein kinase. The present results suggest that in rat liver dibutyryl cAMP modulates delta-aminolevulinate synthase mRNA biosynthesis by acting predominantly, if not exclusively, at the level of gene transcription.

引用

页码：381 / 390

页数：10

共 55 条

[1] BIOGENESIS OF MITOCHONDRIAL PROTEINS REGULATION OF MATURATION OF DELTA-AMINOLEVULINATE SYNTHASE BY HEMIN [J].