ELECTROPHORETIC BEHAVIOR OF A GROUP OF ORGANIC-ANIONS OF BIOCHEMICAL INTEREST IN A FUNCTIONALLY COHERENT SERIES OF BUFFERS

The electrophoretic behaviour of a test group of organic anions of biochemical interest in a functionally coherent series of buffers has been studied concurrently with the associated electroosmotic flow in silica capillaries. Changes in the electroosmotic flow with pH are correlated with probable changes in the silica surface. The variation of electroosmotic and electrophoretic behaviour with capillary age, buffer molarity and ionic strength is studied and results tested against theory. The properties of 15-crown-5 as an electroosmotic flow modifier are described and theoretical implications discussed. Electroosmotic and electrophoretic velocities are linear with electric field strength and capillary current up to 526 V/cm. Analyte peak areas and heights are linear with analyte concentration.