SUPERCOILED PLASMID DNA AS A MODEL TARGET FOR ASSESSING THE GENERATION OF FREE-RADICALS AT THE SURFACE OF FIBERS

被引：4

作者：

DONALDSON, K

GILMOUR, PS

BESWICK, PH

机构：

[1] Department of Biological Sciences, Napier University, Edinburgh

来源：

EXPERIMENTAL AND TOXICOLOGIC PATHOLOGY | 1995年 / 47卷 / 04期

关键词：

DNA; PLASMID; PLASMID DNA; SUPERCOILED PLASMID DNA; FREE RADICALS; RADICALS; FREE; FIBERS; SURFACE; ASBESTOS; CERAMIC FIBERS; VITREOUS FIBERS; CROCIDOLITE ASBESTOS; AMOSITE ASBESTOS; AMPHIBOLE ASBESTOS; MAN MADE FIBERS;

D O I：

10.1016/S0940-2993(11)80255-1

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

The ability of respirable amosite and crocidolite asbestos, refractory ceramic fibres (RCFs) and man made vitreous fibres (MMVFs) to cause free radical injury to plasmid, phi X174 RFI DNA was assessed. The amosite and crocidolite asbestos caused substantial damage to the DNA and, in the main, the free radicals responsible for the asbestos-mediated DNA damage were hydroxyl radicals as bi determined by inhibition with mannitol. Asbestos fibre-mediated damage to the DNA was completely ameliorated by the chelation of fibre-associated iron by pre-treatment of fibres with desferrioxamine-B, confirming the importance of iron in the production of free radicals. MMVFs and RCFs produced modest free radical damage to the DNA, which was prevented by mannitol but not by iron chelation.

引用

页码：235 / 237

页数：3