THROMBOSPONDIN-1 BINDS TO THE SURFACE OF BOVINE ARTICULAR CHONDROCYTES BY A LINEAR RGD-DEPENDENT MECHANISM

被引：6

作者：

MILLER, RR ^{[1
]}

MCDEVITT, CA ^{[1
]}

机构：

[1] CLEVELAND CLIN FDN,RES INST,DEPT BIOMED ENGN,MUSCULOSKELETAL BIOL WB3 SECT,CLEVELAND,OH 44195

来源：

FEBS LETTERS | 1995年 / 363卷 / 03期

关键词：

THROMBOSPONDIN; CHONDROCYTE; CARTILAGE; CELL ATTACHMENT;

D O I：

10.1016/0014-5793(95)00311-V

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Thrombospondin 1 is present in articular cartilage and is synthesized by chondrocytes. Adult bovine articular chondrocytes in serum-free medium were evaluated in a solid-phase assay for their ability to attach to thrombospondin 1 isolated from human platelets. The chondrocytes attached to the thrombospondin 1 by a mechanism that was inhibited by a synthetic linear GRGDSP but not a GRGESP peptide, The cells, however, did not spread on thrombospondin 1, but did spread on fibronectin and Pep-Tite 2000, a synthetic RGD-containing peptide, Preincubation of thrombospondin 1 with EDTA irreversibly inhibited its capacity to attach to chondrocytes. We conclude that thrombospondin 1 binds to chondrocytes by its RGD sequence.

引用

页码：214 / 216

页数：3

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