APPLICATION OF A THERMAL INSITU REACTION FOR FLUORIMETRIC DETECTION OF CARBOHYDRATES ON NH2-LAYERS

被引：25

作者：

KLAUS, R ^{[1
]}

FISCHER, W ^{[1
]}

HAUCK, HE ^{[1
]}

机构：

[1] E MERCK AG,W-6100 DARMSTADT,GERMANY

来源：

CHROMATOGRAPHIA | 1990年 / 29卷 / 9-10期

关键词：

Carbohydrates; Fluorescence detection; NH[!sub]2[!/sub]-layers; Thin-layer chromatography;

D O I：

10.1007/BF02261396

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Based on experience in the purely thermally induced (no analytical reagents) fluorimetric detection of glucose and fructose after thin-layer chromatographic separation on NH2-layers, the application of this method to other sugars is described. After separation with mobile phases containing pyridine, this "dry"in situ derivatization permits a very simple qualitative and quantitative method of detection for the 20 carbohydrates studied. © 1990 Friedr. Vieweg & Sohn Verlagsgesellschaft mbH.

引用

页码：467 / 472

页数：6

共 9 条

[1] A MODIFIED HIGH-PERFORMANCE THIN-LAYER PLATE FOR THE SEPARATION OF PURINES AND PYRIMIDINES [J].