RADIOIMMUNOASSAY OF PREGNANEDIOL-3-ALPHA-GLUCURONIDE

An antibody was prepared against pregnanediol-3α-glucuronide-BSA. The hapten (5β-pregnane-20α-ol-3α-yl-glucuronide) used for the preparation of the immunogen and as 'cold' standard for RIA was synthesized by an unambiguous chemical synthesis. The corresponding [6,7-3H]-labelled conjugate was prepared and the cross-reactions of the antiserum against other glucuronides and free steroids were examined. Application of RIA to menstrual cycle urines and pregnancy urine is discussed. The results of our own studies carried out throughout the menstrual cycle from seven women showed the mean and range for the follicular phase were 3.8 ± 0.67 (3.0 - 5.4) μmol/24 h and for the luteal phase 17.7 ± 6.9 (6.9 - 29.0) μmol/24h. © 1979.