POLYCLONAL AND MONOCLONAL-ANTIBODIES TO FIELD AND STORAGE FUNGI

被引:11
作者
BANKS, JN
COX, SJ
NORTHWAY, BJ
机构
[1] Central Science Laboratory, Ministry of Agriculture, Fisheries and Food, Slough, SL3 7HJ, London Road
关键词
D O I
10.1016/0964-8305(93)90046-5
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A polyclonal antibody (PAb) and a monoclonal antibody (MAb) were raised against Penicillium aurantiogriseum var. melanoconidium. Cross-reactivity studies were carried out against 10 'field' and 27 'storage' fungi. The PAb and MAb cross-reacted with 33 and 37 of these fungi, respectively. Enzyme-linked immunosorbent assay (ELISA) using these antibodies on extracts of barley inoculated with the homologous antigen, showed a clear relationship between absorbance and amount of fungal growth. It is suggested that these antibodies could be used in a broad-spectrum assay to determine the fungal content in feeds and food.
引用
收藏
页码:137 / 144
页数:8
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