ALLERGENS OF PITYROSPORUM-OVALE AND CANDIDA-ALBICANS .1. CROSS-REACTIVITY OF IGE-BINDING COMPONENTS

被引:47
作者
DOEKES, G [1 ]
VANLEPERENVANDIJK, AG [1 ]
机构
[1] UNIV HOSP UTRECHT,DEPT DERMATOL,3508 GA UTRECHT,NETHERLANDS
关键词
ALLERGENS; ATOPIC DERMATITIS; CANDIDA; IGE; PITYROSPORUM;
D O I
10.1111/j.1398-9995.1993.tb00736.x
中图分类号
R392 [医学免疫学];
学科分类号
100102 ;
摘要
In atopic dermatitis (AD), a high prevalence has been reported of type I reactions and specific IgE to extracts of the commensal lipophilic skin yeast Pityrosporum ovale. In the present study, a highly significant correlation (r = 0.77) was found between levels of anti-P. ovale IgE and of IgE reacting with extracts of Candida albicans, both measured by a sensitive ELISA method. In a series of 128 AD sera, 34 sera reacted positively with both yeast extracts, 38 reacted with P. ovale but not with C. albicans, and only one of the 56 anti-P. ovale-negative sera showed a very weak reaction with C. albicans. The correlation was due to a marked cross-reactivity, as shown by inhibition ELISA. Fluid-phase preincubation of double-positive sera with either of the two yeast extracts resulted in a dose-dependent, and at high concentrations complete, inhibition of the IgE reactions with both coated P. ovale and C. albicans allergens. Mutual inhibition of IgE-binding could also be achieved with pools of glycoproteins and/or polysaccharides isolated from the crude extracts by Con A affinity chromatography. P. ovale allergens were, however, more potent fluid-phase inhibitors than the corresponding C. albicans components. The apparently higher avidity for P. ovale allergens suggests that these antiyeast IgE antibodies in AD result from sensitization to P. ovale and cross-react with C. albicans.
引用
收藏
页码:394 / 400
页数:7
相关论文
共 30 条
  • [1] RELATIONSHIP BETWEEN THE RESULTS OF SKIN, CONJUNCTIVAL AND BRONCHIAL TESTS AND RAST WITH CANDIDA-ALBICANS IN PATIENTS WITH ASTHMA
    AKIYAMA, K
    YUI, Y
    SHIDA, T
    MIYAMOTO, T
    [J]. CLINICAL ALLERGY, 1981, 11 (04): : 343 - 351
  • [2] BRUIJNZEELKOOME.CA, 1988, ALLERGY S5, V43, P15
  • [3] CLEMMENSEN OJ, 1983, SEMIN DERMATOL, V2, P26
  • [4] ALLERGENS OF PITYROSPORUM-OVALE AND CANDIDA-ALBICANS .2. PHYSICOCHEMICAL CHARACTERIZATION
    DOEKES, G
    KAAL, MJH
    VANLEPERENVANDIJK, AG
    [J]. ALLERGY, 1993, 48 (06) : 401 - 408
  • [5] ANTIBODIES IN DIFFERENT IMMUNOGLOBULIN CLASSES TO CANDIDA-ALBICANS IN ALLERGIC RESPIRATORY-DISEASE
    EDGE, G
    PEPYS, J
    [J]. CLINICAL ALLERGY, 1980, 10 (01): : 47 - 58
  • [6] FAERGEMANN J, 1983, ACTA DERM-VENEREOL, V63, P346
  • [7] CHALLENGE REACTIONS IN ATOPIC-DERMATITIS AFTER PERCUTANEOUS ENTRY OF MITE ANTIGEN
    GONDO, A
    SAEKI, N
    TOKUDA, Y
    [J]. BRITISH JOURNAL OF DERMATOLOGY, 1986, 115 (04) : 485 - 493
  • [8] GUMOWSKI P, 1987, ANN ALLERGY, V59, P48
  • [9] HANIFIN JM, 1980, ACTA DERM-VENEREOL, V92, P44, DOI [DOI 10.2340/00015555924447, 10.2340/00015555924447]
  • [10] BRONCHIAL HYPERSENSITIVITY TO EXTRACT OF CANDIDA ALBICANS
    ITKIN, IH
    DENNIS, M
    [J]. JOURNAL OF ALLERGY, 1966, 37 (04): : 187 - &