A GOAT-ANTI-HUMAN IGG MODIFIED PIEZOIMMUNOSENSOR FOR STAPHYLOCOCCUS-AUREUS DETECTION

被引:45
作者
LE, D [1 ]
HE, FJ [1 ]
JIANG, TJ [1 ]
NIE, LH [1 ]
YAO, SZ [1 ]
机构
[1] HUNAN UNIV,DEPT CHEM & CHEM ENGN,CHANGSHA 410083,PEOPLES R CHINA
基金
美国国家科学基金会;
关键词
PIEZOELECTRIC IMMUNOSENSOR; STAPHYLOCOCCUS-AUREUS; ANTIBODY; ANTIGEN;
D O I
10.1016/0167-7012(95)00018-G
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Determination of Staphylococcus aureus was performed by using a piezoelectric crystal immunosensor system. The system consists of an oscillator, a frequency counter, a special cell and a modified piezoelectric crystal. Goat-anti-human IgG is immobilized on the surface of the crystal, which is used as a S. aureus detector by measuring its resonant frequency shift due to a mass change caused by specific binding of the microbe to the crystal surface. The frequency shift was correlated with a S. aureus concentration in the range of 5 x 10(5) -1 x 10(8) cells. The coated crystal was stable for fifteen assays without detectable loss of sensitivity and stable for a month when it was stored at + 4 degrees C.
引用
收藏
页码:229 / 234
页数:6
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