EFFECTS OF STROMA-FREE HEMOGLOBIN-SOLUTIONS ON ISOLATED PERFUSED RABBIT HEARTS AND ISOLATED PERFUSED RAT KIDNEYS

被引：22

作者：

VOGEL, WM

LIEBERTHAL, W

APSTEIN, CS

LEVINSKY, N

VALERI, CR

机构：

[1] BOSTON UNIV, SCH MED, CARDIAC MUSCLE RES LAB, BOSTON, MA 02118 USA

[2] BOSTON UNIV HOSP, RENAL SECT, BOSTON, MA 02218 USA

来源：

BIOMATERIALS ARTIFICIAL CELLS AND ARTIFICIAL ORGANS | 1988年 / 16卷 / 1-3期

关键词：

Hemoglobin Solutions SFH was prepared in other laboratories by various methods. We measured hemoglobin concentration, P50, percent carboxy-and methemoglobin, pH, and electrolytes (II), but did not further characterize the purity and composition of the solutions. SFH was prepared from outdated human red cells unless otherwise noted. Unmodified SFH prepared by acid precipitation of lipoproteins and ultrafiltration (17,18), pyridoxalated SFH (18), and glutaraldehyde cross-linked pyridoxalated SFH (19) were provided by Drs. G.S. Moss and L.R. Sehgal, Department of Surgery, Michael Reese Hospital and Medical Center, Chicago, IL. Unmodified SFH crystallized by phosphate precipitation (20) was provided by Dr. F. DeVenuto, Petaluma, CA. Unmodified SFH prepared by compartmentalization and ultrafiltration (21) and glyoxalated glutaraldehyde cross-linked SFH (22.23) were provided by Drs. R. Condie and B.E. Hedlund, Departments of Surgery and Biochemistry, University of Minnesota, Minneapolis, MN. Lightly glutaraldehyde cross-linked SFH (24) was provide by Drs. W. Ottinger and D.H. Marks, Clinical Research Laboratory, Keesler USAF Medical Center, Keesler AFB, MS. SFH purified by ATP-agarose affinity chromatography (25) was provided by Dr. J.C. Hsia, Defence and Civil Institute of Environmental Medicine, Downsview, Ontario, Canada. Hemoglobin Ao prepared by ion-exchange chromatography (26) was provided by Dr. M. Farmer, Naval Research Laboratory, Washington, DC. Unmodified bovine SFH prepared by ultrafiltration and preparative HPLC, and glutaraldehyde cross-linked bovine SFH were provided by Dr. K. Rausch, BIOPURE Corporation, Boston, MA;

D O I：

10.3109/10731198809132572

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

引用

页码：227 / 235

页数：9

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