ISOLATION AND GENETIC CHARACTERISTICS OF LAMBDA TRANSDUCING PHAGES OF THE UVRA+ AND UVRC+ GENES OF E-COLI-K12

被引：10

作者：

AUERBACH, J

HOWARDFLANDERS, P

机构：

[1] YALE UNIV,SCH MED,DEPT PSYCHIAT,NEW HAVEN,CT 06520

[2] YALE UNIV,SCH MED,DEPT THERAPEUT RADIOL,NEW HAVEN,CT 06520

来源：

MOLECULAR & GENERAL GENETICS | 1979年 / 168卷 / 03期

关键词：

D O I：

10.1007/BF00271506

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Lambda transducing phages carying the excision repair genes uvrA+ and uvrC+ were selected from a pool of λ phages carrying EcoR1 fragments of E. coli DNA. These phages and also λuvrB+ (obtained from Gottesman) were used to make lysogens of excision-defective strains carrying uvrA-, uvrB- or uvrC-. Lambda uvrA+ was found to transduce strains carrying uvrA- but not those carrying uvrB- or uvrC-, to normal ultraviolet resistance. Similarly, λuvrB+ and λuvrC+ were found to complement only the corresponding uvr- allele. The λ transducing phages were co-transduced with gal+ by P1 phage into lysogenic gal- recipients, and presumably were integrated at the normal prophage site. © 1979 Springer-Verlag.

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页码：341 / 344

页数：4

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