共 32 条
METABOLISM OF HYDROXYBIPHENYL AND CHLORO-HYDROXYBIPHENYL BY BIPHENYL/CHLOROBIPHENYL DEGRADING PSEUDOMONAS-TESTOSTERONI, STRAIN B-356
被引:31
作者:
SONDOSSI, M
[1
]
SYLVESTRE, M
[1
]
AHMAD, D
[1
]
MASSE, R
[1
]
机构:
[1] UNIV QUEBEC,INST NATL RECH SCI SANTE,POINTE CLAIRE H9R 1G6,QUEBEC,CANADA
来源:
JOURNAL OF INDUSTRIAL MICROBIOLOGY
|
1991年
/
7卷
/
02期
关键词:
PCB DEGRADATION;
COMMON PATHWAY;
BIPHENYL;
HYDROXYBIPHENYL;
CHLORO-HYDROXYBIPHENYL;
CHLOROBIPHENYL;
PSEUDOMONAS-TESTOSTERONI;
PSEUDOMONAS-PUTIDA;
D O I:
10.1007/BF01576069
中图分类号:
Q81 [生物工程学(生物技术)];
Q93 [微生物学];
学科分类号:
071005 ;
0836 ;
090102 ;
100705 ;
摘要:
A biphenyl (BP) and chlorobiphenyl (CBP) metabolizing Pseudomonas testosteroni, strain B-356 was also capable of utilizing 2-,3-, and 4-hydroxybiphenyl. Data presented here suggest that utilization of biphenyl and mono-substituted biphenyls involves the enzymes of the same pathway. Chloro-hydroxybiphenyls were also metabolized by strain B-356. The unsubstituted ring is first hydroxylated in position 2 and 3 and then cleaved in a meta 1, and 2, position to ultimately generate the benzoic acid derivatives. Since strain B-356 was capable of utilizing benzoic acid and mono-hydroxybenzoic acids, the utilization of biphenyl, 2-,3-, and 4-hydroxybiphenyl is complete at non-toxic concentrations of the substrates. Chlorobenzoic acids and chloro-hydroxybenzoic acids were not metabolized further by this strain. Studies using Pseudomonas putida, strain KT2440 carrying cloned BP/CBP genes from strain B-356 provided further evidence for the presence of a common pathway for the metabolism of the above compounds in P. testosteroni, strain B-356. Suggestions are made on significance of the broad substrate specificity of the enzymes of biphenyl/chlorobiphenyl pathway in regard to their possible origin and in relation to PCB mixture degradation.
引用
收藏
页码:77 / 88
页数:12
相关论文