PHENOTYPIC PLASTICITY OF AVIAN EMBRYONIC SYMPATHETIC NEURONS GROWN IN A CHEMICALLY DEFINED MEDIUM - DIRECT EVIDENCE FOR NORADRENERGIC AND CHOLINERGIC PROPERTIES IN THE SAME NEURONS

被引:7
作者
BARBU, M [1 ]
POURQUIE, O [1 ]
VAIGOT, P [1 ]
GATEAU, G [1 ]
SMITH, J [1 ]
机构
[1] COLL FRANCE, F-94736 NOGENT SUR MARNE, FRANCE
关键词
CATECHOLAMINES; ACETYLCHOLINE; CHOLINE ACETYLTRANSFERASE; TYROSINE HYDROXYLASE; FLUORESCENCE-ACTIVATED CELL SORTING; SIF CELLS;
D O I
10.1002/jnr.490320307
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Avian embryonic sympathetic ganglia possess both catecholaminergic and cholinergic features and can synthesize noradrenaline (NAd) and acetylcholine (ACh) simultaneously. In the present study we sought to determine (1) whether or not this coproduction of NAd and ACh corresponds to the existence of two non-overlapping populations, and (2) to what extent the levels of synthesis are influenced by non-neuronal ganglion cells. We have focused on the correlation between the immunocytochemically demonstrable presence of the noradrenergic and cholinergic enzymes tyrosine hydroxylase (TH) and choline acetyltransferase (ChAT), respectively, and the synthesis of the corresponding neurotransmitters in embryonic quail sympathetic neuronal and non-neuronal cells purified by fluorescence-activated cell sorting. We show that (1) freshly sorted neurons synthesize both NAd and ACh, whereas non-neuronal cells produce neither; (2) the overwhelming majority of the sympathetic neurons display TH immunoreactivity; (3) about half of the TH-positive neurons are recognized by an anti-ChAT antibody in an artificial medium that selectively enhances synthesis and/or accumulation of ACh; (4) the non-neuronal cells are important for survival of the neurons and potentiate their synthesis of ACh in this medium, and (5) finally, we present evidence that expression of TH in noradrenergic neurons and in small intensely fluorescent cells of sympathetic ganglia is differentially regulated.
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页码:350 / 362
页数:13
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