CAPILLARY GEL-ELECTROPHORESIS OF SINGLE-STRANDED-DNA FRAGMENTS WITH UV DETECTION

被引：19

作者：

HUANG, XC ^{[1
]}

STUART, SG ^{[1
]}

BENTE, PF ^{[1
]}

BRENNAN, TM ^{[1
]}

机构：

[1] GENOMYX INC,S SAN FRANCISCO,CA 94080

来源：

JOURNAL OF CHROMATOGRAPHY | 1992年 / 600卷 / 02期

关键词：

D O I：

10.1016/0021-9673(92)85560-G

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Capillary gel electrophoresis has proven to be a powerful tool in biomedical research. We report our investigation of some of the critical parameters affecting separations of single-stranded DNA fragments as monitored by ultraviolet (UV) absorbance detection. Although not as sensitive as laser-induced fluorescence (LIF), UV absorbance detection allows one to calculate quite accurately, and inexpensively, the molarity of each separated DNA fragment and, moreover, the signal "fading" effect normally observed with LIF detection can be, in many cases, substituted for fluorescence to detect the many different single-stranded DNAs, as well as for detection of sequencing reactions.

引用

页码：289 / 295

页数：7