DIFFERENT MODULATORY EFFECTS OF ELEVATED CYCLIC-AMP ON CYTOSOLIC CA2+ SPIKES INDUCED BY PHENYLEPHRINE OR VASOPRESSIN IN SINGLE-RAT HEPATOCYTES

被引：29

作者：

SANCHEZBUENO, A ^{[1
]}

MARRERO, I ^{[1
]}

COBBOLD, PH ^{[1
]}

机构：

[1] UNIV LIVERPOOL, DEPT HUMAN ANAT & CELL BIOL, POB 147, LIVERPOOL L69 3BX, ENGLAND

来源：

BIOCHEMICAL JOURNAL | 1993年 / 291卷

基金：

英国惠康基金;

关键词：

D O I：

10.1042/bj2910163

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We show here, by aequorin measurements in single isolated rat hepatocytes, that elevation of cyclic AMP, by dibutyryl cyclic AMP, forskolin or glucagon, has different effects on oscillations in cytosolic concentration of free Ca2+ ('free Ca') induced by phenylephrine or vasopressin. Elevated cyclic AMP does not itself induce free Ca oscillations, but enhances both the peak free Ca and the frequency of spikes induced by phenylephrine. In contrast, elevated cyclic AMP has no effect on peak free Ca of vasopressin-induced spikes, but markedly prolongs the falling phase, with the result that spiking frequency (peak to peak) falls, although the period between spikes of resting free Ca is usually decreased. The data provide another example of receptor-specific information being retained in the oscillator mechanism, with implications for models of the hepatocyte calcium oscillator.

引用

页码：163 / 168

页数：6

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