CLONING AND EXPRESSION OF A HUMAN GLUCAGON RECEPTOR

被引：68

作者：

MACNEIL, DJ

OCCI, JL

HEY, PJ

STRADER, CD

GRAZIANO, MP

机构：

[1] Department of Molecular Pharmacology and Biochemistry, Merck Research Laboratories, Rahway

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 198卷 / 01期

关键词：

D O I：

10.1006/bbrc.1994.1046

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A human glucagon receptor has been cloned from human liver tissue. The 1578-bp cDNA clone encodes a protein of 477 amino acids with 82% identity to the rat glucagon receptor. The predicted secondary structure and homology to known proteins places this receptor within the superfamily of seven transmembrane domain G protein coupled receptors. Transfection of the human glucagon receptor into COS-7 cells confers upon them high affinity binding for [125I] glucagon. In membranes prepared from COS-7 cells transfected with the human glucagon receptor, the binding of [125I] glucagon is inhibited with the rank order of potency glucagon > oxyntomodulin > glucagon-like peptide 1 (7-36) amide ≫ glucagon-like peptide 2 = gastric inhibitory peptide = secretin. © 1994 Academic Press, Inc.

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收藏

页码：328 / 334

页数：7

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