PRODUCTION OF XYLANOLYTIC ENZYMES DURING GROWTH ON PULVERIZED GRASS BY ASPERGILLUS OCHRACEUS-42

被引:9
作者
DAS, A [1 ]
NANDA, G [1 ]
机构
[1] BOSE INST,DEPT MICROBIOL,CALCUTTA 700054,W BENGAL,INDIA
关键词
D O I
10.1111/j.1472-765X.1995.tb00411.x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Xylanase and beta-xylosidase with activity of 6.46 U mg(-1) and 0.500 U mg(-1), respectively, were produced extracellularly by Aspergillus ochraceus during growth on pulverized grass in liquid state fermentation, compared to 9.3 U mg(-1) and 0.74 U mg(-1) when pure xylan was used. The culture filtrate was devoid of any cellulase activity. Xylanolytic enzymes were produced optimally in 144 h of incubation on 1% pulverized grass, pH 6.5. About 8.43% (w/w) sugars were liberated from alkali-treated grass in 6 h by the synergistic effect of xylanolytic enzymes. The half-lives for xylanase and beta-xylosidase at 50 degrees C were 210 min and 300 min, respectively, and half-life increased with the increase in protein concentration. Both mono- and divalent cations, especially K+ and Zn2+, exhibited a profound effect on the rate of enzyme saccharification.
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页码:141 / 144
页数:4
相关论文
共 11 条
[1]  
BALL AS, 1988, J GEN MICROBIOL, V134, P2139
[2]   XYLANASE AND BETA-XYLOSIDASE PRODUCTION BY ASPERGILLUS-OCHRACEUS DURING GROWTH ON LIGNOCELLULOSES [J].
BISWAS, SR ;
MISHRA, AK ;
NANDA, G .
BIOTECHNOLOGY AND BIOENGINEERING, 1988, 31 (06) :613-616
[3]   PRODUCTION OF XYLANASES BY CHAETOMIUM-CELLULOLYTICUM DURING GROWTH ON LIGNOCELLULOSES [J].
DUBEAU, H ;
CHAHAL, DS ;
ISHAQUE, M .
BIOTECHNOLOGY LETTERS, 1986, 8 (06) :445-448
[4]   RELATIONSHIP BETWEEN AGE AND PROPERTIES OF HUMAN AND RABBIT ERYTHROCYTE GLUCOSE-6-PHOSPHATE DEHYDROGENASE [J].
FORNAINI, G ;
LEONCINI, G ;
SEGNI, P ;
CALABRIA, GA ;
DACHA, M .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1969, 7 (02) :214-+
[5]  
HERBERT D, 1971, METHODS MICROBIOLO B, V5, P289
[6]  
LOWRY OH, 1951, J BIOL CHEM, V193, P265
[7]   STUDIES ON THE EXTRACELLULAR XYLANASE ACTIVITY OF SOME THERMOPHILIC ACTINOMYCETES [J].
MCCARTHY, AJ ;
PEACE, E ;
BRODA, P .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1985, 21 (3-4) :238-244
[8]  
Rydholm S. A, 1965, PULPING PROCESSING, P95
[9]  
SOMOGYI M, 1952, J BIOL CHEM, V4, P341