MICROELECTROPHORETIC INVESTIGATION OF RNA BASE COMPOSITION IN NERVE CELLS OF N SUPRAOPTICUS IN ALBINO RAT AT DIFFERENT STAGES OF ESTROUS CYCLE

Female rats of the Wistar strain were killed by decapitation in the stages of estrus and diestrus. RNA content was measured, and its base composition was determined by the microelectrophoresis method of Edström in neurosecretory cells of N. supraopticus of the hypothalamus. Cells were isolated from Carnoy-fixed tissue. Each sample consisted of 10-15 isolated cells. Significant differences between the average RNA content per nerve cell in estrus and diestrus were not observed (in estrus it was equal to 70.3±5.52 and in diestrus 88.4±9.14 picograms per cell). The adenine/uracil ratio was higher in diestrous stage than in estrus (1.35±0.16 and 0.88±0.05). © 1968 Plenum Publishing Corporation.