STUDIES ON STEROIDS .140. DETERMINATION OF ESTROGEN RING-D-GLUCURONIDES IN PREGNANCY PLASMA BY DIRECT RADIOIMMUNOASSAY WITHOUT HYDROLYSIS

被引：18

作者：

NUMAZAWA, M ^{[1
]}

TANAKA, T ^{[1
]}

NAMBARA, T ^{[1
]}

机构：

[1] TOHOKU UNIV,INST PHARMACEUT,SENDAI,MIYAGI 980,JAPAN

来源：

CLINICA CHIMICA ACTA | 1979年 / 91卷 / 02期

关键词：

D O I：

10.1016/0009-8981(79)90453-4

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

A direct radioimmunoassay method using highly specific antisera without prior deconjugation has been developed for determination of estradiol 17-glucuronide and estriol 16-glucoronide in human plasma. Antisera were elicited in the rabbit by immunization with antigens in which the steroid haptens are linked to a carrier protein through the C-2 or C-4 position. After treatment with Rivanol the protein of albumin-free antiserum was covalently bound to a p-arylamine glass bead support through the cross-linkage with glutaraldehyde. A simple and reliable assay method employing the antibody-glass preparation was established and applied to measurement of estrogen ring D glucuronide concentration in peripheral plasma throughout normal pregnancy. © 1979.

引用

页码：169 / 175

页数：7

共 14 条

[1] SOLID-PHASE RADIOIMMUNOASSAY OF ESTRADIOL-17BETA [J].