MOLECULAR-CLONING, SEQUENCE, AND TISSUE DISTRIBUTION OF THE HUMAN UBIQUITIN-ACTIVATING ENZYME-E1

被引：122

作者：

HANDLEY, PM

MUECKLER, M

SIEGEL, NR

CIECHANOVER, A

SCHWARTZ, AL

机构：

[1] WASHINGTON UNIV,SCH MED,DEPT PHARMACOL,ST LOUIS,MO 63110

[2] WASHINGTON UNIV,SCH MED,DEPT CELL BIOL,ST LOUIS,MO 63110

[3] MONSANTO CO,ST LOUIS,MO 63166

[4] TECHNION ISRAEL INST TECHNOL,SCH MED,DEPT BIOCHEM,HAIFA,ISRAEL

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1991年 / 88卷 / 01期

关键词：

PROTEIN DEGRADATION;

D O I：

10.1073/pnas.88.1.258

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The ubiquitin-activating enzyme E1 catalyzes the first step in ubiquitin conjugation. We have cloned and sequenced the cDNA for human E1. This clone predicts a protein of 110,450 Da. Cys-194 lies within a region of identity to active-site Cys-88 of the ubiquitin carrier protein E2, suggesting a potential role for this region in enzymatic function of this protein. In addition, Cys-454 lies within a region of identity to the thiol ester consensus sequence of several proteins involved in thioester formation. Tissue distribution reveals a single 3.5-kilobase E1 message ubiquitous among tissues and cell lines.

引用

页码：258 / 262

页数：5

共 26 条

[1] INTERNAL AMINO-ACID SEQUENCE-ANALYSIS OF PROTEINS SEPARATED BY ONE-DIMENSIONAL OR TWO-DIMENSIONAL GEL-ELECTROPHORESIS AFTER INSITU PROTEASE DIGESTION ON NITROCELLULOSE [J].