USE OF INEXPENSIVE O-ANTISERA AS THE DETECTING ANTIBODIES FOR SALMONELLA-ANTIGENS IN THE POLYMYXIN-CLOTH ENZYME-IMMUNOASSAY

被引:14
作者
BLAIS, BW
ONG, HY
YAMAZAKI, H
机构
[1] CARLETON UNIV,DEPT BIOL,OTTAWA K1S 5B6,ONTARIO,CANADA
[2] CARLETON UNIV,INST BIOCHEM,OTTAWA K1S 5B6,ONTARIO,CANADA
关键词
SALMONELLA; SEROGROUP; O-ANTISERA; POLYMYXIN-CLOTH ENZYME IMMUNOASSAY;
D O I
10.1016/0168-1605(93)90107-R
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
The application of O antigen-specific antisera to the detection of Salmonella lipopolysaccharide (LPS) antigens was examined in an enzyme immunoassay using polymyxin-coated polyester cloth. The LPS antigens were extracted with deoxycholate and captured on polymyxin-cloth. A mixture of rabbit antisera to Salmonella O antigens was allowed to react with the captured antigens, and the reacted antibodies were detected by an anti-rabbit IgG-peroxidase conjugate. The assay gave positive results with 40 different Salmonella serotypes which represented more than 99.9% of the serogroups isolated from over 122000 food, feed and environmental samples analysed at Laboratory Services Division, Agriculture Canada, from 1975 to 1990. Strong cross-reactivity with Staphylococcus aureus was eliminated by pregrowth of the organisms in the presence of sodium deoxycholate. The O antisera were commercially available and are more economical than monoclonal or affinity purified polyclonal antibodies.
引用
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页码:149 / 158
页数:10
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