A RECOMBINATION-BASED ASSAY DEMONSTRATES THAT THE FRAGILE-X SEQUENCE IS TRANSCRIBED WIDELY DURING DEVELOPMENT

被引：18

作者：

HANZLIK, AJ ^{[1
]}

OSEMLAKHANZLIK, MM ^{[1
]}

HAUSER, MA ^{[1
]}

KURNIT, DM ^{[1
]}

机构：

[1] UNIV MICHIGAN,SCH MED,HOWARD HUGHES MED INST,ANN ARBOR,MI 48109

来源：

NATURE GENETICS | 1993年 / 3卷 / 01期

关键词：

D O I：

10.1038/ng0193-44

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

To identify transcribed sequences rapidly and efficiently, we have developed a recombination-based assay to screen bacteriophage lambda libraries for sequences that share homology with a given probe. This strategy determines analytically whether a given probe is transcribed in a given tissue at a given time of development, and may also be used to isolate preparatively the transcribed sequence free of the screening probe. We illustrate this technology for the fragile X sequence, demonstrating that it is transcribed ubiquitously in an 11 week fetus, in a variety of 20 week human fetal tissues, including brain, spinal cord, eye, liver, kidney and skeletal muscle, and in adult jejunum.

引用

页码：44 / 48

页数：5

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