DENATURATION MAP OF BACTERIOPHAGE P1 DNA

被引:17
作者
MEYER, J
STALHAMMARCARLEMALM, M
IIDA, S
机构
关键词
D O I
10.1016/0042-6822(81)90018-0
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
EM of bacteriophage P1 plasmid DNA partially denatured by high pH in the presence of formaldehyde and mounted by the protein monolayer technique revealed a unique denaturation pattern. Alignment with the restriction cleavage map was obtained by comparing the melting pattern of plasmid DNA linearized by a single cut introduced by the restriction enzyme PstI with those of the large HindIII, BglII and BamHI restriction fragments. Major A + T-rich segments center around map units 3, 21, 46 and 52. Other denatured regions are located around map units 29, 32 (within the invertible segment), 62, 88 and 96. The invertible segment of P1 DNA was precisely mapped within the BamHI-5 and the BglII-5 DNA restriction fragments. The DNA segment melting first and therefore having the highest A + T content spans the P1-specific restriction modification region between map units 0-6 of the P1 genome. The P1-specific recombination site, loxP, is also located in a region of low DNA helix stability. The denaturation pattern of the DNA of a hybrid P1-R plasmid revealed IS1-mediated transposition of the r-determinant into a site adjacent to the most A + T-rich region on P1.
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页码:167 / 175
页数:9
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