DETERMINATION OF BASE PAIRING CONTENT OF RIBONUCLEIC ACIDS BY INFRARED SPECTROSCOPY

被引：61

作者：

THOMAS, GJ

机构：

[1] Medical Research Council Biophysics Research Unit, King's College, Department of Biophysics, London

[2] Department of Chemistry, Southeastern Massachusetts Technological Institute, North Dartmouth, Massachusetts

来源：

BIOPOLYMERS | 1969年 / 7卷 / 03期

关键词：

D O I：

10.1002/bip.1969.360070305

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A method is described for determining the fractions of adenine‐uracil and guanine‐cytosine base pairs of partly double‐helical ribonucleic acids in aqueous solution. The method is based upon the ability to distinguish between paired and unpaired bases by means of infrared spectroscopy of their deuterium oxide solutions in the 1500–1800 cm−1 frequency region of the infrared spectrum. An application of the method to yeast ribosomal RNA is described. At 30°C, ribosomal RNA is 64± 6% paired (35% GC, 29% AU). The method can be applied to determine the fractions of Watson‐Crick base pairs at a given temperature in any RNA containing the four common bases in known ratios. Copyright © 1969 John Wiley & Sons, Inc.

引用

页码：325 / &

共 37 条

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