SIMPLE TECHNIQUE FOR DETECTION AND QUANTITATION OF LACTOSE SYNTHESIS AND SECRETION

被引：4

作者：

EMERMAN, JT

BISSELL, MJ

机构：

[1] Laboratory of Chemical Biodynamics, Lawrence Berkeley Laboratory, University of California, Berkeley

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 94卷 / 02期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0003-2697(79)90370-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A combination of high specific activity [14C]glucose, two-dimensional chromatography, and autoradiography can be utilized to follow the flow of glucose carbon into lactose and various intermediary metabolites of the mammary gland. The technique described requires a minimum amount of tissue manipulation, is quantitative, utilizes microgram quantities of sample, and can be used for tissue pieces, single cell suspensions, and cells in culture. Clean separation of lactose from intermediates of glucose metabolism in a single chromatogram will aid the study of the regulation of mammary-specific functions. © 1979.

引用

页码：340 / 345

页数：6

共 24 条

[1]

ABRAHAM S, 1964, J BIOL CHEM, V239, P855

[2]

ABRAHAM S, 1959, J BIOL CHEM, V234, P2246

[3] OXIDATION AND UTILIZATION OF PALMITATE STEARATE OLEATE AND ACETATE BY MAMMARY GLAND OF FED GOAT IN RELATION TO THEIR OVERALL METABOLISM AND ROLE OF PLASMA PHOSPHOLIPIDS AND NEUTRAL LIPIDS IN MILK-FAT SYNTHESIS [J].