RAPID SEPARATION OF BOVINE BETA-CRYSTALLIN SUBUNITS-BETA-B1, SUBUNIT-BETA-B2, SUBUNIT-BETA-B3, SUBUNIT-BETA-A3 AND SUBUNIT-BETA-A4

被引：65

作者：

SLINGSBY, C

BATEMAN, OA

机构：

[1] Birkbeck College, Laboratory of Molecular Biology, Department of Crystallography, London, WC1E 7HX, Malet Street

来源：

EXPERIMENTAL EYE RESEARCH | 1990年 / 51卷 / 01期

关键词：

eye lens proteins; Fast Protein Liquid Chromatography System; βH; βL1; βL2-crystallins;

D O I：

10.1016/0014-4835(90)90165-Q

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

Bovine β-crystallin aggregates, βH-, βL1- and βL2-crystallins, prepared by rapid gel filtration, are each subjected to anion-exchange chromatography in deaggregating media using a Pharmacia Fast Protein Liquid Chromatography System. BetaB1, βB2 and βA4 subunits are rapidly isolated using a one step Mono Q column from βH-, βL2- and βL1-crystallin, respectively. BetaB3 and βA3 are separated from each other using a second Mono Q column starting from βL2- and βL1-crystallin respectively. Whereas βB2, βB3 and βA4 are common to all sizes of aggregate, βB1 is restricted to βH-crystallin and βA3 is absent from βL2-crystallin. © 1990.

引用

页码：21 / 26

页数：6

共 19 条

[1] IMPROVED RESOLUTION OF CALF LENS BETA-CRYSTALLINS
ASSELBERGS, FAM
KOOPMANS, M
VANVENROOIJ, WJ
BLOEMENDAL, H
[J]. EXPERIMENTAL EYE RESEARCH, 1979, 28 (02) : 223 - 228
[2] PROLINE-RICH AND ALANINE-RICH N-TERMINAL EXTENSION OF THE BASIC BOVINE BETA-CRYSTALLIN B1-CHAINS
BERBERS, GAM
HOEKMAN, WA
BLOEMENDAL, H
DEJONG, WW
KLEINSCHMIDT, T
BRAUNITZER, G
[J]. FEBS LETTERS, 1983, 161 (02) : 225 - 229
[3] HOMOLOGY BETWEEN THE PRIMARY STRUCTURES OF THE MAJOR BOVINE BETA-CRYSTALLIN CHAINS
BERBERS, GAM
HOEKMAN, WA
BLOEMENDAL, H
DEJONG, WW
KLEINSCHMIDT, T
BRAUNITZER, G
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1984, 139 (03): : 467 - 479
[4] BERBERS GAM, 1982, EUR J BIOCHEM, V128, P495
[5] STRUCTURAL ASPECTS OF BOVINE BETA-CRYSTALLINS - PHYSICAL CHARACTERIZATION INCLUDING DISSOCIATION-ASSOCIATION BEHAVIOR
BINDELS, JG
KOPPERS, A
HOENDERS, HJ
[J]. EXPERIMENTAL EYE RESEARCH, 1981, 33 (03) : 333 - 343
[6] RECOMMENDATIONS FOR CRYSTALLIN NOMENCLATURE
BLOEMENDAL, H
PIATIGORSKY, J
SPECTOR, A
[J]. EXPERIMENTAL EYE RESEARCH, 1989, 48 (04) : 465 - 466
[7] de Jong W.W, 1981, MOL CELLULAR BIOL EY, P221, DOI DOI 10.1146/ANNUREV.ECOLSYS.33.020602.095451
[8] PRIMARY STRUCTURE OF THE BOVINE BETA-CRYSTALLIN BP CHAIN - INTERNAL DUPLICATION AND HOMOLOGY WITH GAMMA-CRYSTALLIN
DRIESSEN, HP
HERBRINK, P
BLOEMENDAL, H
DEJONG, WW
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1981, 121 (01): : 83 - 91
[9] CLONING AND CHARACTERIZATION OF A COW BETA-CRYSTALLIN CDNA
GORIN, MB
HORWITZ, J
[J]. CURRENT EYE RESEARCH, 1984, 3 (07) : 939 - 948
[10] Harding JJ, 1984, EYE, V16, P207

← 1 2 →