ASSEMBLY OF THE SEA-URCHIN EXTRAEMBRYONIC HYALINE LAYER - CA2+ AND MG2+ ACT INDEPENDENTLY AND AT DIFFERENT SITES ON THE PATHWAY LEADING TO HYALIN-GEL FORMATION

被引:9
作者
ROBINSON, JJ
BRENNAN, C
机构
[1] Department of Biochemistry, Memorial University of Newfoundland, St. John's
关键词
D O I
10.1016/0003-9861(91)90361-L
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have studied the interactions of Ca2+ with the sea urchin extraembryonic coat protein hyalin. As reported previously, Ca2+ alone was ineffective in inducing hyalin-gel (large aggregate) formation. This reaction required the additional presence of Mg2+ and NaCl. However, the results of tryptic digestion and nondenaturing agarose gel electrophoresis experiments demonstrated that Ca2+ could induce hyalin self-association into small aggregates in the absence of Mg2+ and NaCl. Magnesium did not modulate the interactions of Ca2+ with hyalin. In addition, Mg2+ had minimal effects on the conformation of hyalin. These results have been incorporated into a model delineating the pathway leading to hyalin-gel formation. © 1991.
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页码:285 / 290
页数:6
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