A SYNTHETIC OLIGOPEPTIDE DERIVED FROM HUMAN THYROTROPIN RECEPTOR SEQUENCE BINDS TO GRAVES IMMUNOGLOBULIN AND INHIBITS THYROID STIMULATING ANTIBODY-ACTIVITY BUT LACKS INTERACTIONS WITH TSH

被引：58

作者：

MORI, T ^{[1
]}

SUGAWA, H ^{[1
]}

PIRAPHATDIST, T ^{[1
]}

INOUE, D ^{[1
]}

ENOMOTO, T ^{[1
]}

IMURA, H ^{[1
]}

机构：

[1] KYOTO UNIV,SCH MED,DIV INTERNAL MED 2,KYOTO 60601,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 178卷 / 01期

关键词：

D O I：

10.1016/0006-291X(91)91794-D

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An 11-residue oligopeptide, P-195, was synthesized to match human thyrotropin (TSH) receptor structure from No. 333 to 343 of amino acid sequence. Preincubation of 5 Graves' IgGs with P-195 up to 10 μg resulted in dose-dependent reductions of thyroid stimulating antibody (TSAb) activity. [125I] labeled P-195 was found to bind Graves' IgG. The bound radioactivity correlated significantly with their TSAb activity (N=25, r=0.587, p<0.01). A peptide having a completely reverse sequence as P-195 did not show such biological activity. The peptide did not affect TSH and thyrotropin binding inhibitor immunoglobulin (TBII) on their receptor binding nor biological activities. P-195 was concluded to have a part of TSAb binding sites. © 1991.

引用

页码：165 / 172

页数：8

共 19 条

[1] CLONING, CHROMOSOMAL ASSIGNMENT, AND REGULATION OF THE RAT THYROTROPIN RECEPTOR - EXPRESSION OF THE GENE IS REGULATED BY THYROTROPIN, AGENTS THAT INCREASE CAMP LEVELS, AND THYROID AUTOANTIBODIES
AKAMIZU, T
IKUYAMA, S
SAJI, M
KOSUGI, S
KOZAK, C
MCBRIDE, OW
KOHN, LD
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (15) : 5677 - 5681
[2] ROLE OF THYROTROPIN RECEPTOR ANTIBODIES IN THE DEVELOPMENT OF HYPERTHYROIDISM - FOLLOW-UP STUDIES ON 9 PATIENTS WITH GRAVES-DISEASE
KASAGI, K
TAMAI, H
MORITA, T
HIDAKA, A
HATABU, H
MISAKI, T
IIDA, Y
ISHIHARA, T
IKEKUBO, K
KUMA, K
KONISHI, J
[J]. JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1989, 68 (06) : 1189 - 1194
[3] MULTICOMPONENT STRUCTURE OF THE THYROTROPIN RECEPTOR - RELATIONSHIP TO GRAVES-DISEASE
KOHN, LD
VALENTE, WA
LACCETTI, P
COHEN, JL
ALOJ, SM
GROLLMAN, EF
[J]. LIFE SCIENCES, 1983, 32 (1-2) : 15 - 30
[4] MECHANISMS BY WHICH LOW SALT CONDITION INCREASES SENSITIVITY OF THYROID STIMULATING ANTIBODY-ASSAY
KOSUGI, S
MORI, T
IMURA, H
[J]. ENDOCRINOLOGY, 1989, 125 (01) : 410 - 417
[5] CLONING, SEQUENCING AND EXPRESSION OF THE HUMAN THYROTROPIN (TSH) RECEPTOR - EVIDENCE FOR BINDING OF AUTOANTIBODIES
LIBERT, F
LEFORT, A
GERARD, C
PARMENTIER, M
PERRET, J
LUDGATE, M
DUMONT, JE
VASSART, G
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 165 (03) : 1250 - 1255
[6] CLONING AND SEQUENCING OF PORCINE LH-HCG RECEPTOR CDNA - VARIANTS LACKING TRANSMEMBRANE DOMAIN
LOOSFELT, H
MISRAHI, M
ATGER, M
SALESSE, R
VU, MT
THI, HL
JOLIVET, A
GUIOCHONMANTEL, A
SAR, S
JALLAL, B
GARNIER, J
MILGROM, E
[J]. SCIENCE, 1989, 245 (4917) : 525 - 528
[7] CLONING, SEQUENCING AND EXPRESSION OF HUMAN TSH RECEPTOR
MISRAHI, M
LOOSFELT, H
ATGER, M
SAR, S
GUIOCHONMANTEL, A
MILGROM, E
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1990, 166 (01) : 394 - 403
[8] MORI T, 1986, CLIN ENDOCRINOL TOKY, V34, P859
[9] IDENTIFICATION OF IMMUNOGENIC REGIONS IN HUMAN THYROTROPIN RECEPTOR FOR IMMUNOGLOBULIN-G OF PATIENTS WITH GRAVES-DISEASE
MURAKAMI, M
MORI, M
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1990, 171 (01) : 512 - 518
[10] THYROTROPIN LUTEINIZING-HORMONE CHORIONIC-GONADOTROPIN RECEPTOR EXTRACELLULAR DOMAIN CHIMERAS AS PROBES FOR THYROTROPIN RECEPTOR FUNCTION
NAGAYAMA, Y
WADSWORTH, HL
CHAZENBALK, GD
RUSSO, D
SETO, P
RAPOPORT, B
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (03) : 902 - 905

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