THE PROTOONCOGENES C-FOS AND C-JUN MODULATE THYROID-HORMONE INHIBITION OF HUMAN THYROTROPIN-BETA SUBUNIT GENE-EXPRESSION IN OPPOSITE DIRECTIONS

被引：5

作者：

BODENNER, DL

MCCLASKEY, JH

KIM, MK

MIXSON, AJ

WEINTRAUB, BD

机构：

[1] Molecular and Cellular Endocrinology Branch (MCEB), National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 189卷 / 02期

关键词：

D O I：

10.1016/0006-291X(92)92310-T

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The sequence from -1 to +6 bp in the hTSHβ gene contains overlapping putative thyroid hormone and AP-1 response elements. We demonstrate interaction between the AP-1 constituents c-fos and c-jun and thyroid hormone receptor in this region by transient transfection experiments using a -125 to +37 bp hTSHβ fragment. T3 inhibition was completely abolished by c-jun, but increased threefold by c-fos. A single transversion mutation at +2 bp restored T3 inhibition in the presence of c-jun and markedly reduced binding of purified c-jun by gel mobility shift assay. Thus, c-fos and c-jun influence T3 inhibition of hTSHβ expression in opposite directions acting through a response element shared with thyroid hormone receptor. Control of the relative cellular levels of these two proto-oncogenes may play a major role in modulating thyroid hormone inhibitory responses. © 1992.

引用

页码：1050 / 1056

页数：7

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