FLAGELLUM-MEDIATED ADHESION OF TRYPANOSOMA-CONGOLENSE TO BOVINE AORTA ENDOTHELIAL-CELLS

被引:33
作者
HEMPHILL, A [1 ]
ROSS, CA [1 ]
机构
[1] UNIV EDINBURGH,CTR TROP & VET MED,EDINBURGH EH25 9RG,MIDLOTHIAN,SCOTLAND
来源
PARASITOLOGY RESEARCH | 1995年 / 81卷 / 05期
关键词
D O I
10.1007/BF00931503
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
We studied the interaction between Trypanosoma congolense and bovine aorta endothelial (BAE) cell monolayers. Our findings suggest that trypanosomes adhere predominantly to the flattened, peripheral cell surface domains as well as to filamentous endothelial outgrowths that are present during in vitro cultivation in non-confluent monolayers. Adhesion is mediated exclusively by the flagellum in a distinct geometrical order with respect to the flagellar cytoskeleton. Thus, it is possible to define exactly the trypanosomal cell surface domain involved in the attachment process. After 24-48 h of cultivation on monolayers, trypanosomes start to develop short, filopodia-like flagellar protrusions, which serve as additional elements in assisting parasite attachment. Small filaments (3-5 nm) also serve as cross-links between flagelIar and endothelial cell surface membranes. Lectin-gold labeling shows that these cross-links contain sialic acid residues. In vitro assays confirm that sialic acid is involved in the adhesion process, whereas the extracellular matrix (ECM) proteins fibronectin, collagen, laminin and vitronectin are not. The presence of T. congolense exhibits a mitogenic effect on BAE cells.
引用
收藏
页码:412 / 420
页数:9
相关论文
共 37 条
[1]   INJURY INDUCED BY TRYPANOSOMA-CONGOLENSE ADHESION TO CELL-MEMBRANES [J].
BANKS, KL .
JOURNAL OF PARASITOLOGY, 1980, 66 (01) :34-37
[2]   BINDING OF TRYPANOSOMA-CONGOLENSE TO WALLS OF SMALL BLOOD-VESSELS [J].
BANKS, KL .
JOURNAL OF PROTOZOOLOGY, 1978, 25 (02) :241-245
[3]   INVITRO BINDING OF TRYPANOSOMA-CONGOLENSE TO ERYTHROCYTES [J].
BANKS, KL .
JOURNAL OF PROTOZOOLOGY, 1979, 26 (01) :103-108
[4]  
Bray D., 1992, CELL MOVEMENTS
[5]  
BRODEN A, 1904, B SOC ETUD COLON BRU
[6]  
BUENGENER W, 1976, Tropenmedizin und Parasitologie, V27, P370
[7]   CELL-ADHESION AND THE MOLECULAR PROCESSES OF MORPHOGENESIS [J].
EDELMAN, GM .
ANNUAL REVIEW OF BIOCHEMISTRY, 1985, 54 :135-169
[8]  
Ellen Richard P., 1994, Trends in Microbiology, V2, P114, DOI 10.1016/0966-842X(94)90597-5
[9]   SIALIDASES FROM AFRICAN TRYPANOSOMES [J].
ENGSTLER, M ;
SCHAUER, R .
PARASITOLOGY TODAY, 1993, 9 (06) :222-225
[10]   TRANS-SIALIDASES IN THE INSECT-VECTOR STAGES OF AFRICAN AND AMERICAN TRYPANOSOMES [J].
FRASCH, ACC .
PARASITOLOGY TODAY, 1994, 10 (05) :170-171