HIGH ALPHA-2,6-SIALYLATION OF N-ACETYLLACTOSAMINE SEQUENCES IN RAS-TRANSFORMED RAT FIBROBLASTS CORRELATES WITH HIGH INVASIVE POTENTIAL

被引：47

作者：

LEMARER, N ^{[1
]}

STEHELIN, D ^{[1
]}

机构：

[1] INST PASTEUR,MOLEC ONCOL LAB,CNRS,URA 1160,F-59019 LILLE,FRANCE

来源：

GLYCOBIOLOGY | 1995年 / 5卷 / 02期

关键词：

ADHESION; INVASION; RAS ONCOGENE; SIALYLATION;

D O I：

10.1093/glycob/5.2.219

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Through cloning experiments with the FRras EJ4 cell line, previously described to exhibit a Sambucus nigra agglutinin (SNA)+ phenotype, three clones with a SNA- phenotype were isolated, All the selected SNA+ and SNA- clones expressed the ras oncoprotein and cloned in soft agar with the same efficiency. We were interested in studying the adhesion and invasion properties of the FRras cells presenting a SNA + or - phenotype. They were first compared in their biochemical properties and we found that FRras SNA- were characterized by a low alpha-2,6-sialylation of their cell surface glycoproteins and a low beta-galactoside alpha-2,6 sialyltransferase activity. Using in vitro invasion assays, FRras cells exhibiting a low alpha-2,6-sialylation on their surface were found to have a low invasive potential compared to their counterpart SNA+. FRras SNA- clones exhibit a different morphology from that of FRras SNA+ clones, Moreover, homotypic aggregation assays indicated that FRras SNA- were more cohesive with each other and adhesion assays showed that they were more adhesive to fibronectin.

引用

页码：219 / 226

页数：8

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