ANALYSIS OF MURINE ANTIBODY-RESPONSES TO BACULOVIRUS-EXPRESSED HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 ENVELOPE GLYCOPROTEINS

被引：8

作者：

BRISTOW, RGW ^{[1
]}

DOUGLAS, AR ^{[1
]}

SKEHEL, JJ ^{[1
]}

DANIELS, RS ^{[1
]}

机构：

[1] NATL INST MED RES, DIV VIROL, LONDON NW7 1AA, ENGLAND

来源：

JOURNAL OF GENERAL VIROLOGY | 1994年 / 75卷

关键词：

D O I：

10.1099/0022-1317-75-8-2089

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An analysis of the humoral immunogenicity of a candidate AIDS vaccine (VaxSyn) in a murine model system is presented. Sera taken from a panel of mice immunized with the immunogen were analysed for their ability to bind a panel of gp120-representing peptides and limited reactivity to known sites of immunological interest was observed. Monoclonal antibodies (MAbs) were characterized as binding to a more restricted variety of regions on gp 120 including C1, V2, V4 and the C terminus. A tetrazolium-based cytocidicity assay was used and shown to be an effective and objective method for the screening of human immunodeficiency virus (HIV)-neutralizing activity in large numbers of samples. None of the MAbs characterized in this study neutralize HIV-1 reference strains. The significance of these findings in view of previous publications is discussed.

引用

页码：2089 / 2095

页数：7

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