THE PHYSIOLOGICAL-ROLE OF INDOLE ACETIC-ACID IN THE MOSS FUNARIA-HYGROMETRICA HEDW .1. QUANTIFICATION OF INDOLE-3-ACETIC-ACID IN TISSUE AND PROTOPLASTS BY ENZYME-IMMUNOASSAY AND GAS-CHROMATOGRAPHY MASS-SPECTROMETRY

被引：11

作者：

ATZORN, R ^{[1
]}

GEIER, U ^{[1
]}

SANDBERG, G ^{[1
]}

机构：

[1] SWEDISH UNIV AGR SCI, DEPT FOREST GENET & PLANT PHYSIOL, S-90183 UMEA, SWEDEN

来源：

JOURNAL OF PLANT PHYSIOLOGY | 1990年 / 135卷 / 05期

关键词：

BHT; Butyl hydroxy toluene; EIA; enzyme immunoassay; Enzyme immunoassay; Funaria hygrometrica; gas chromatography-mass spectrometry; Gas chromatography-mass spectrometry; GC-MS; High performance liquid chromatography; HPLC; IAA; IAAme; Indole-3-acetic acid; indole-3-acetic acid; Methyl ester of indole-3-acetic acid; Polyvinylpyrrolidone; protoplasts; PVP;

D O I：

10.1016/S0176-1617(11)80628-0

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A procedure for extraction, purification and quantification by reversed-phase high-performance liquid chromatography (HPLC) — enzyme immunoassay (EIA) was developed which allowed the specific and reliable determination of indole-3-acetic acid (IAA) contents in tissue and protoplasts of protonema of the moss Funaria hygrometrica Hedw. The results were validated by gas chromatography-mass spectrometry (GC-MS). © 1990, Gustav Fischer Verlag, Stuttgart. All rights reserved.

引用

页码：522 / 525

页数：4

共 12 条

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