DETERMINATION OF ERYTHROMYCIN IN HUMAN PLASMA AND WHOLE-BLOOD BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引:11
作者
KATO, Y
YOKOYAMA, T
SHIMOKAWA, M
KUDO, K
KABE, J
MOHRI, K
机构
[1] NATL MED CTR HOSP,DEPT INTERNAL MED,SHINJUKU KU,TOKYO 162,JAPAN
[2] MEIJI COLL PHARM,DEPT BIOPHARM,TOKYO 154,JAPAN
来源
JOURNAL OF LIQUID CHROMATOGRAPHY | 1993年 / 16卷 / 03期
关键词
D O I
10.1080/10826079308019556
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A simple, selective, sensitive, and rapid high-performance liquid chromatographic (HPLC) method is described for the quantitation of erythromycin (EM) in plasma and whole blood. The volume of plasma and whole blood and analytical time were only 0.2 ml and approximately 15 min. Analytical recovery of EM at concentrations of 0.2, 1.0 and 5.0 mug/ml after the complete extraction procedures with diethyl ether from alkalinized plasma and whole blood was > 75%. The coefficients of variation (CVs) for intra- and inter- day assay riabilities at 0.2, 1.0 and 5.0 mug/ml of EM were < 5.8% in plasma and < 6.3% in whole blood, respectively. The calibration curves for the respective analyte were linear (r = 0.994) over concentrations ranging from 0.1 to 5.0 mug/ml in plasma and whole blood. The lower detection limit for EM in plasma and whole blood was 0.1 mug/ml (CVs < 5.3%). This assay method is useful for the routine monitoring of EM in biological specimens.
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页码:661 / 680
页数:20
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