HEPARIN INHIBITS ENDOTHELIN-1 AND PROTOONCOGENE C-FOS GENE-EXPRESSION IN CULTURED BOVINE ENDOTHELIAL-CELLS

被引：13

作者：

IMAI, T ^{[1
]}

HIRATA, Y ^{[1
]}

MARUMO, F ^{[1
]}

机构：

[1] TOKYO MED & DENT UNIV, DEPT INTERNAL MED 2,DIV ENDOCRINE HYPERTENS, YUSHIMA 1-5-45,BUNKYO KU, TOKYO 113, JAPAN

来源：

JOURNAL OF CARDIOVASCULAR PHARMACOLOGY | 1993年 / 22卷

关键词：

ENDOTHELIN-1; THROMBIN; HEPARIN; MESSENGER RNA INDUCTION; C-FOS; PROTOONCOGENE; ENDOTHELIAL CELLS;

D O I：

10.1097/00005344-199322008-00015

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

We studied the inhibitory effects of heparin, thrombin inhibitor, and protein kinase C (PKC) inhibitor on basal and thrombin-induced preproendothelin-1 (pre-pro-ET-1) and proto-oncogene c-fos mRNA expression in cultured bovine endothelial cells (ECs). Northern blot analysis using cDNA for bovine prepro-ET-1 as a probe showed that heparin lowered not only the basal but also the stimulated expression of prepro-ET-1 mRNA by thrombin. A selective thrombin inhibitor (argatroban) and a PKC inhibitor (staurosporine) also inhibited thrombin-induced but not basal prepro-ET-1 mRNA expression. Heparin similarly inhibited thrombin-induced c-fos proto-oncogene mRNA expression in ECs. These data suggest that heparin, in addition to its antithrombin effect, has an inhibitory effect on prepro-ET-1 mRNA expression, possibly via a PKC-dependent pathway.

引用

页码：S49 / S52

页数：4

共 18 条

[1]

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