RETICULOCYTE MEASUREMENTS AS A BIOASSAY FOR ERYTHROPOIETIN

被引：9

作者：

BARBONE, AG ^{[1
]}

APARICIO, B ^{[1
]}

ANDERSON, DW ^{[1
]}

NATARAJAN, J ^{[1
]}

RITCHIE, DM ^{[1
]}

机构：

[1] RW JOHNSON PHARMACEUT RES INST,LA JOLLA,CA 92037

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 1994年 / 12卷 / 04期

关键词：

RETICULOCYTE; ERYTHROPOIETIN; FLOW CYTOMETRY; BIOASSAY;

D O I：

10.1016/0731-7085(93)E0006-9

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The possibility of using reticulocyte counts in peripheral blood to assay erythropoietic activity of recombinant human erythropoietin (r-HuEPO) was evaluated in normal mice. Mice were injected subcutaneously with r-HuEPO on days 0, 1 and 2 and bled on day 4 for reticulocyte count determinations, using an automated counting system with thiazole orange fluorescent staining and flow cytometric analysis. Reticulocyte counts increased in a dose-dependent fashion upon administration of r-HuEPO. The reticulocyte count was unaffected by asialylated EPO as well as other substances tested (interleukin-1, interleukin-3, dexamethasone, human chorionic gonadotropin). These data demonstrate the usefulness of employing reticulocyte counts as a rapid, specific and reproducible assay for in vivo erythropoietic activity of r-HuEPO.

引用

页码：515 / 522

页数：8

共 22 条

[1] ABELS RI, 1991, BLOOD CELL GROWTH FACTORS : THEIR PRESENT AND FUTURE USE IN HEMATOLOGY AND ONCOLOGY, P121
[2] BARNETT V, 1978, OUTLIERS STATISTICAL
[3] ERYTHROPOIETIN - GENE CLONING, PROTEIN-STRUCTURE, AND BIOLOGICAL PROPERTIES
BROWNE, JK
COHEN, AM
EGRIE, JC
LAI, PH
LIN, FK
STRICKLAND, T
WATSON, E
STEBBING, N
[J]. COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY, 1986, 51 : 693 - 702
[4] BIO-ASSAY OF ERYTHROPOIETIN IN MICE MADE POLYCYTHAEMIC BY EXPOSURE TO AIR AT A REDUCED PRESSURE
COTES, PM
BANGHAM, DR
[J]. NATURE, 1961, 191 (479) : 1065 - &
[5] HUMAN IL-3 AND GM-CSF ACT SYNERGISTICALLY IN STIMULATING HEMATOPOIESIS IN PRIMATES
DONAHUE, RE
SEEHRA, J
METZGER, M
LEFEBVRE, D
ROCK, B
CARBONE, S
NATHAN, DG
GARNICK, M
SEHGAL, PK
LASTON, D
LAVALLIE, E
MCCOY, J
SCHENDEL, PF
NORTON, C
TURNER, K
YANG, YC
CLARK, SC
[J]. SCIENCE, 1988, 241 (4874) : 1820 - 1823
[6] A DOSE-DEPENDENT EFFECT OF RECOMBINANT ERYTHROPOIETIN ON THE RETICULOCYTE POPULATION OF RATS
EDER, H
ROSSLENBROICH, B
FAILING, K
[J]. BLUT, 1989, 59 (02): : 184 - 187
[7] DEVELOPMENT OF RADIOIMMUNOASSAYS FOR HUMAN ERYTHROPOIETIN USING RECOMBINANT ERYTHROPOIETIN AS TRACER AND IMMUNOGEN
EGRIE, JC
COTES, PM
LANE, J
DAS, REG
TAM, RC
[J]. JOURNAL OF IMMUNOLOGICAL METHODS, 1987, 99 (02) : 235 - 241
[8] Finney D, 1978, STATISTICAL METHOD B, P69
[9] FUKADA MN, 1989, BLOOD, V73, P84
[10] ERYTHROPOIETIN AND CONTROL OF RED-CELL PRODUCTION
GRABER, SE
KRANTZ, SB
[J]. ANNUAL REVIEW OF MEDICINE, 1978, 29 : 51 - 66

← 1 2 3 →