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A MAP OF THE DISTAL REGION OF THE LONG ARM OF HUMAN CHROMOSOME-21 CONSTRUCTED BY RADIATION HYBRID MAPPING AND PULSED-FIELD GEL-ELECTROPHORESIS

被引：135
作者：
BURMEISTER, M
KIM, SW
PRICE, ER
DELANGE, T
TANTRAVAHI, U
MYERS, RM
COX, DR
机构：
[1] UNIV CALIF SAN FRANCISCO, SCH MED, DEPT PSYCHIAT, SAN FRANCISCO, CA 94143 USA
[2] UNIV CALIF SAN FRANCISCO, SCH MED, DEPT BIOCHEM & BIOPHYS, SAN FRANCISCO, CA 94143 USA
[3] UNIV CALIF SAN FRANCISCO, SCH MED, DEPT MICROBIOL & IMMUNOL, SAN FRANCISCO, CA 94143 USA
[4] HARVARD UNIV, SCH MED, CHILDRENS HOSP, DIV GENET, BOSTON, MA 02115 USA
来源：
GENOMICS | 1991年 / 9卷 / 01期
关键词：
D O I：
10.1016/0888-7543(91)90216-2
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
We have used radiation hybrid (RH) mapping and pulsedfield gel electrophoresis (PFGE) to determine the order and positions of 28 DNA markers from the distal region of the long arm of human chromosome 21. The maps generated by these two methods are in good agreement. This study, combined with that of D. R. Cox et al. (1990, Science 250:245-250), results in an RH map that covers the long arm of chromosome 21 (21q). We have used a subtelomeric probe to show that our map includes the telomere and have identified single-copy genes and markers within 200 kbp of the telomere. Comparison of the physical and RH maps with genetic linkage maps shows "hot spots" of meiotic recombination in the distal region, one of which is close to the telomere, in agreement with previous cytogenetic observations of increased recombination frequency near telomeres. © 1991.
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收藏
页码：19 / 30
页数：12
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