SIMULTANEOUS DETERMINATION OF DIAZEPAM AND ITS METABOLITES IN PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：9

作者：

FERNANDEZ, P

HERMIDA, I

BERMEJO, AM

LOPEZRIVADULLA, M

CRUZ, A

CONCHEIRO, L

机构：

[1] Department of Legal Medicine, Service of Forensic Toxicology, Faculty of Medicine, University of Santiago de Compostela

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1991年 / 14卷 / 13期

关键词：

D O I：

10.1080/01483919108049340

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A reversed phase high-performance liquid chromatographic method (HPLC) for the simultaneous determination of diazepam and its three active metabolites, nordiazepam, oxazepam and temazepam, in plasma was proposed. The compounds were isolated by solid-phase extraction. The chromatographic mobile phase was metanol-water (55:45, v/v) at a flow rate of 1 mL/min. UV detection was performed concurrently at 240 and 254 nm.

引用

页码：2587 / 2599

页数：13

共 15 条

[1] CORRECTED RETENTION INDEXES IN HPLC - THEIR USE FOR THE IDENTIFICATION OF ACIDIC AND NEUTRAL-DRUGS [J].

BOGUSZ, M ;

ADERJAN, R .

JOURNAL OF ANALYTICAL TOXICOLOGY, 1988, 12 (02) :67-72

[2]

DROVETCOASSOLO C, 1989, J CHROMATOGR, V487, P295

[3]

ELLENHORN MJ, 1988, MED TOXICOLOGY DIAGN, P580

[4] ELECTRON-CAPTURE GAS-CHROMATOGRAPHY DETERMINATION OF BENZODIAZEPINES AND METABOLITES [J].

FERGUSON, JL ;

COURI, D .

JOURNAL OF ANALYTICAL TOXICOLOGY, 1977, 1 (04) :171-174

[5]

GOTTSCHALK LA, 1980, TOXICOLOGICAL PATHOL

[6] COLLECTION OF ANALYTICAL DATA FOR BENZODIAZEPINES AND BENZOPHENONES [J].

JAPP, M ;

GARTHWAITE, K ;

GEESON, AV ;

OSSELTON, MD .

JOURNAL OF CHROMATOGRAPHY, 1988, 439 (02) :317-339

[7]

KARNES HT, 1987, LC GC-MAG SEP SCI, V5, P978

[8] SIMULTANEOUS DETERMINATION OF DIAZEPAM AND ITS ACTIVE METABOLITES IN RAT SERUM, BRAIN AND CEREBROSPINAL-FLUID BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

KLOCKOWSKI, PM ;

LEVY, G .

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1987, 422 :334-339

[9] MICROSAMPLE DETERMINATION OF DIAZEPAM AND ITS 3 METABOLITES IN SERUM BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

LAU, CE ;

DOLAN, S ;

TANG, M .

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1987, 416 (01) :212-218

[10] USE OF INTERNAL STANDARDS IN ESTIMATION OF DIAZEPAM IN PLASMA [J].

MCALLISTER, CB .

JOURNAL OF CHROMATOGRAPHY, 1978, 151 (01) :62-66

← 1 2 →