PRETRANSFUSED PATIENTS WITH SEVERE APLASTIC-ANEMIA EXHIBIT HIGH NUMBERS OF CYTOTOXIC LYMPHOCYTE-T PRECURSORS PROBABLY DIRECTED AT NON-HLA ANTIGENS

被引:25
作者
KAMINSKI, ER
HOWS, JM
GOLDMAN, JM
BATCHELOR, JR
机构
[1] HAMMERSMITH HOSP, ROYAL POSTGRAD MED SCH, DEPT IMMUNOL, LONDON W12 0HS, ENGLAND
[2] HAMMERSMITH HOSP, ROYAL POSTGRAD MED SCH, DEPT HAEMATOL, LONDON W12 0HS, ENGLAND
关键词
D O I
10.1111/j.1365-2141.1990.tb06375.x
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Patients with severe aplastic anaemia (SAA) have a relatively high risk of graft rejection after transplantation of bone marrow from HLA‐identical siblings compared with patients transplanted for leukaemia. This is presumed to be due to pretransplant blood transfusions which sensitize the recipient's immune system to non‐HLA antigens expressed on donor marrow cells. To test this hypothesis, we estimated in the peripheral blood of 18 SAA patients, the frequencies of alloreactive cytotoxic T lymphocyte precursors (CTL‐p) directed against mononuclear cells from syngeneic twins, HLA‐identical siblings or HLA‐matched unrelated individuals. The results were compared with the frequencies of CTL‐p in 13 healthy subjects or leukaemia patients who had not been transfused. Pretransfused patients with SAA had significantly higher frequencies of CTL‐p directed against HLA‐identical siblings (P= 0·002), and against HLA‐matched unrelated individuals (P= 0·004), than did untransfused individuals. In contrast, the frequency of CTL‐p in two pretransfused patients against syngeneic mononuclear cells was very low. We propose that the increased numbers of CTL‐p in the blood of pretransfused SAA patients are directed against non‐HLA antigens on target cells and may be the result of prior blood transfusion. Copyright © 1990, Wiley Blackwell. All rights reserved
引用
收藏
页码:401 / 405
页数:5
相关论文
共 28 条
  • [1] A DNA-RFLP TYPING SYSTEM THAT POSITIVELY IDENTIFIES SEROLOGICALLY WELL-DEFINED AND ILL-DEFINED HLA-DR AND DQ ALLELES, INCLUDING DRW10
    BIDWELL, JL
    BIDWELL, EA
    SAVAGE, DA
    MIDDLETON, D
    KLOUDA, PT
    BRADLEY, BA
    [J]. TRANSPLANTATION, 1988, 45 (03) : 640 - 646
  • [2] REGULATION OF CYTO-TOXIC LYMPHOCYTE-T MEDIATED GRAFT-REJECTION FOLLOWING BONE-MARROW TRANSPLANTATION
    BIERER, BE
    EMERSON, SG
    ANTIN, J
    MAZIARZ, R
    RAPPEPORT, JM
    SMITH, BR
    BURAKOFF, SJ
    [J]. TRANSPLANTATION, 1988, 46 (06) : 835 - 839
  • [3] BOSSERMAN LD, 1989, BONE MARROW TRANSPL, V4, P239
  • [4] DEEG HJ, 1986, BLOOD, V68, P1363
  • [5] DENNERT G, 1985, J IMMUNOL, V135, P3729
  • [6] RECOGNITION OF HUMAN MINOR ALLOANTIGEN(S) BY CYTO-TOXIC LYMPHOCYTES INVITRO
    ELKINS, WL
    PIERSON, G
    NEGENDANK, W
    ZIER, KS
    [J]. IMMUNOGENETICS, 1982, 15 (05) : 485 - 499
  • [7] GOULMY E, 1976, LANCET, V2, P1206
  • [8] GOULMY E, 1985, PROG ALLERGY, V36, P44
  • [9] GOULMY E, 1988, TRANSPLANT REV, V2, P29, DOI DOI 10.1016/S0955-470X(88)80005-3
  • [10] ALLOREACTIVE T-CELL RESPONSES BETWEEN HLA-IDENTICAL SIBLINGS - DETECTION OF ANTI-MINOR HISTOCOMPATIBILITY T-CELL CLONES INDUCED INVIVO
    IRLE, C
    BEATTY, PG
    MICKELSON, E
    THOMAS, ED
    HANSEN, JA
    [J]. TRANSPLANTATION, 1985, 40 (03) : 329 - 333