TISSUE-SPECIFIC SPLICING INVIVO OF THE BETA-TROPOMYOSIN GENE - DEPENDENCE ON AN RNA SECONDARY STRUCTURE

被引：143

作者：

LIBRI, D ^{[1
]}

PISERI, A ^{[1
]}

FISZMAN, MY ^{[1
]}

机构：

[1] DIPARTIMENTO GENET & BIOL MICROORGANISMI, I-20131 MILAN, ITALY

来源：

SCIENCE | 1991年 / 252卷 / 5014期

关键词：

D O I：

10.1126/science.2063196

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The beta-tropomyosin gene in chicken contains two mutually exclusive exons (exons 6A and 6B) which are used by the splicing apparatus in myogenic cells, respectively, before (myoblast stage) and after (myotube stage) differentiation. The myoblast splicing pattern is shown to depend on multiple sequence elements that are located in the upstream intron and in the exon 6B and that exert a negative control over exon 6B splicing. This regulation of splicing is due, at least in part, to a secondary structure of the primary transcript, which limits in vivo the accessibility of exon 6B in myoblasts.

引用

页码：1842 / 1845

页数：4

共 24 条

[1] THE 216-NUCLEOTIDE INTRON OF THE E1A PRE-MESSENGER RNA CONTAINS A HAIRPIN STRUCTURE THAT PERMITS UTILIZATION OF UNUSUALLY DISTANT BRANCH ACCEPTORS [J].

CHEBLI, K ;

GATTONI, R ;

SCHMITT, P ;

HILDWEIN, G ;

STEVENIN, J .

MOLECULAR AND CELLULAR BIOLOGY, 1989, 9 (11) :4852-4861

[2] RNA SECONDARY STRUCTURE REPRESSION OF A MUSCLE-SPECIFIC EXON IN HELA-CELL NUCLEAR EXTRACTS [J].

DORVAL, BC ;

CARAFA, YD ;

SIRANDPUGNET, P ;

GALLEGO, M ;

BRODY, E ;

MARIE, J .

SCIENCE, 1991, 252 (5014) :1823-1828

[3] EFFECTS OF RNA SECONDARY STRUCTURE ON ALTERNATIVE SPLICING OF PRE-MESSENGER RNA - IS FOLDING LIMITED TO A REGION BEHIND THE TRANSCRIBING RNA-POLYMERASE [J].

EPERON, LP ;

GRAHAM, IR ;

GRIFFITHS, AD ;

EPERON, IC .

CELL, 1988, 54 (03) :393-401

[4] THE ROLE OF NUCLEOTIDE-SEQUENCES IN SPLICE SITE SELECTION IN EUKARYOTIC PRE-MESSENGER-RNA [J].

EPERON, LP ;

ESTIBEIRO, JP ;

EPERON, IC .

NATURE, 1986, 324 (6094) :280-282

[5] USE OF RNASE-H AND PRIMER EXTENSION TO ANALYZE RNA SPLICING [J].

ERSTER, SH ;

FINN, LA ;

FRENDEWEY, DA ;

HELFMAN, DM .

NUCLEIC ACIDS RESEARCH, 1988, 16 (13) :5999-6014

[6]

FISZMAN MY, UNPUB

[7] IMPROVED FREE-ENERGY PARAMETERS FOR PREDICTIONS OF RNA DUPLEX STABILITY [J].

FREIER, SM ;

KIERZEK, R ;

JAEGER, JA ;

SUGIMOTO, N ;

CARUTHERS, MH ;

NEILSON, T ;

TURNER, DH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (24) :9373-9377

[8] FACTORS INFLUENCING ALTERNATIVE SPLICE SITE UTILIZATION INVIVO [J].

FU, XY ;

MANLEY, JL .

MOLECULAR AND CELLULAR BIOLOGY, 1987, 7 (02) :738-748

[9] INVITRO SPLICING OF MUTUALLY EXCLUSIVE EXONS FROM THE CHICKEN BETA-TROPOMYOSIN GENE - ROLE OF THE BRANCH POINT LOCATION AND VERY LONG PYRIMIDINE STRETCH [J].

GOUXPELLETAN, M ;

LIBRI, D ;

DAUBENTONCARAFA, Y ;

FISZMAN, M ;

BRODY, E ;

MARIE, J .

EMBO JOURNAL, 1990, 9 (01) :241-249

[10] NEW TECHNIQUE FOR ASSAY OF INFECTIVITY OF HUMAN ADENOVIRUS 5 DNA [J].

GRAHAM, FL ;

VANDEREB, AJ .

VIROLOGY, 1973, 52 (02) :456-467

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