HYDROXYETHYLMETHACRYLATE COLUMN REACTORS WITH IMMOBILIZED GLUCOSE-OXIDASE OR ALCOHOL OXIDASE - LIQUID-CHROMATOGRAPHIC DETERMINATION OF ETHANOL IN SERUM

被引：8

作者：

PACAKOVA, V ^{[1
]}

STULIK, K ^{[1
]}

LE, K ^{[1
]}

HLADIK, J ^{[1
]}

机构：

[1] CHARLES UNIV, DEPT BIOCHEM, CS-12840 PRAGUE 2, CZECHOSLOVAKIA

来源：

ANALYTICA CHIMICA ACTA | 1992年 / 257卷 / 01期

关键词：

AMPEROMETRY; LIQUID CHROMATOGRAPHY; BLOOD; ENZYME REACTOR; ETHANOL; SERUM;

D O I：

10.1016/0003-2670(92)80152-W

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Glucose oxidase and alcohol oxidase were immobilized in column reactors packed with hydroxyethylmethacrylate-based sorbents modified with epoxy or vinylsulphate groups. The properties of the reactors were tested in the determination of glucose and ethanol. The best results were obtained with a sorbent containing a high concentration of epoxy groups (900-1100-mu-mol g-1). The limits of detection were 1.0 x 10(-5) mol l-1 for glucose and 8.6 x 10(-5) mol l-1 for ethanol. The linear dynamic ranges amounted to about three concentration decades. The calibration graphs exhibited a good linearity and the precision of measurement was satisfactory. The method was applied to the determination of ethanol in serum after chromatographic separation on a C18 column.

引用

页码：73 / 78

页数：6

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