MONOKARYOTIZATION BY PROTOPLASTING HETEROTHALLIC SPECIES OF EDIBLE MUSHROOMS

被引：61

作者：

ZHAO, J ^{[1
]}

CHANG, ST ^{[1
]}

机构：

[1] CHINESE UNIV HONG KONG,DEPT BIOL,SHA TIN,HONG KONG

来源：

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 1993年 / 9卷 / 05期

关键词：

BREEDING; EDIBLE MUSHROOM; MONOKARYOTIZATION; PROTOPLAST;

D O I：

10.1007/BF00386290

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 0836 [生物工程]; 090102 [作物遗传育种]; 100705 [微生物与生化药学];

摘要：

After regeneration of protoplasts of seven heterothallic species of edible mushrooms, two types of monokaryons were recovered, being identified by the absence of clamp connections. In regenerated colonies, monokaryons grew more slowly than dikaryons and so could be distinguished from them. In all species, the yield of monokaryons in the regenerated colonies was over 45%. The mating types and auxotrophic markers were the same in the protoplasted monokaryons and parental monokaryons. In comparison with other monokaryotization methods, protoplasting is rapid, simple and effective; its applications in breeding of edible mushrooms are discussed.

引用

页码：538 / 543

页数：6

共 17 条

[1]

Burnett J.H., Aspects of the structure and growth of hyphal walls, Fungal Walls and Hyphal Growth, pp. 1-25, (1979)

[2]

Castle A.J., Horgen P.A., Anderson J.B., Restriction fragment length polymorphisms in the mushrooms Agaricus brunnescens and Agaricus bitorquis, Applied and Environmental Microbiology, 53, pp. 816-822, (1987)

[3]

Chang S.T., Li G.S.F., Peberdy J.F., Isolation of protoplasts from edible fungi, MIRCEN Journal of Applied Microbiology and Biotechnology, 1, pp. 185-193, (1985)

[4]

Kiguchi T., Yanagi O.S., Intraspecific heterokaryon and fruit body formation in Coprinus macrorhizus by protoplast fusion of auxotrophic mutants, Applied Microbiology and Biotechnology, 22, pp. 121-127, (1985)

[5]

Kirimura K., Imura M., Lee S.P., Kato Y., Usami S., Intergenetic hybridization between Aspergillus niger and Trichoderma viride by protoplast fusion, Agricultural and Biological Chemistry, 53, pp. 1589-1596, (1989)

[6]

Leal-Lara H., Eger-Hummel G., A monokaryotization method and its use for genetic studies in wood-rotting Basidiomycetes, Theoretical and Applied Genetics, 61, pp. 65-68, (1982)

[7]

Magae Y., Kakimoto Y., Kashiwagi Y., Sasaki T., Fruit-body formation from regenerated mycelium of Pleurotus ostreatus protoplasts, Applied and Environmental Microbiology, 49, pp. 441-442, (1985)

[8]

Miles P.G., Raper J.R., Recovery of the component strains from dikaryotic mycelia, Mycologia, 48, pp. 484-494, (1956)

[9]

Peberdy J.F., Developments in protoplast fusion in fungi, Microbiological Science, 4, pp. 108-114, (1987)

[10]

Peberdy J.F., Fungi without coats—protoplasts as tools for mycological research, Mycological Research, 93, pp. 1-20, (1989)

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