A STRONG-STOP DNA IN RICE PLANTS INFECTED WITH RICE TUNGRO BACILLIFORM VIRUS

被引：5

作者：

BAO, YM ^{[1
]}

HULL, R ^{[1
]}

机构：

[1] JOHN INNES INST,JOHN INNES CTR PLANT SCI RES,DEPT VIRUS RES,COLNEY LANE,NORWICH NR4 7UH,NORFOLK,ENGLAND

来源：

JOURNAL OF GENERAL VIROLOGY | 1993年 / 74卷

关键词：

D O I：

10.1099/0022-1317-74-8-1611

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A virus-specific small nucleic acid (strong-stop DNA) was identified in rice plants infected with rice tungro bacilliform virus, but not in the virus particles. This nucleic acid was shown to consist of about 595 deoxyribonucleotides with about 70 ribonucleotides covalently linked at the 5' end. Hybridization with sequence-specific oligonucleotides showed that the ribonucleotides were from the plant cytoplasmic tRNA(i)Met sequence. PCR analysis detected hairpin structures at the 3' end of the DNA.

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页码：1611 / 1616

页数：6

相关论文

共 34 条

[1] CHARACTERIZATION OF THE DISCONTINUITIES IN RICE TUNGRO BACILLIFORM VIRUS-DNA [J].

BAO, YM ;

HULL, R .

JOURNAL OF GENERAL VIROLOGY, 1992, 73 :1297-1301

[2] NUCLEOTIDE-SEQUENCES OF THE INITIATOR TRANSFER-RNAS FROM BEAN CYTOPLASM AND CHLOROPLASTS [J].

CANADAY, J ;

GUILLEMAUT, P ;

WEIL, JH .

NUCLEIC ACIDS RESEARCH, 1980, 8 (05) :999-1008

[3]

CONDIT C, 1983, MOL APPLIED GENETICS, V2, P301

[4] HAIRPIN DNAS OF CAULIFLOWER MOSAIC-VIRUS GENERATED BY REVERSE TRANSCRIPTION INVIVO [J].

COVEY, SN ;

TURNER, DS .

EMBO JOURNAL, 1986, 5 (11) :2763-2768

[5] CHARACTERIZATION OF CAULIFLOWER MOSAIC-VIRUS DNA-SEQUENCES WHICH ENCODE MAJOR POLYADENYLATED TRANSCRIPTS [J].

COVEY, SN ;

LOMONOSSOFF, GP ;

HULL, R .

NUCLEIC ACIDS RESEARCH, 1981, 9 (24) :6735-6747

[6] A SMALL DNA MOLECULE CONTAINING COVALENTLY-LINKED RIBONUCLEOTIDES ORIGINATES FROM THE LARGE INTERGENIC REGION OF THE CAULIFLOWER MOSAIC-VIRUS GENOME [J].

COVEY, SN ;

TURNER, D ;

MULDER, G .

NUCLEIC ACIDS RESEARCH, 1983, 11 (02) :251-264

[7] RICE TUNGRO BACILLIFORM VIRUS-DNA INDEPENDENTLY INFECTS RICE AFTER AGROBACTERIUM-MEDIATED TRANSFER [J].

DASGUPTA, I ;

HULL, R ;

EASTOP, S ;

POGGIPOLLINI, C ;

BLAKEBROUGH, M ;

BOULTON, MI ;

DAVIES, JW .

JOURNAL OF GENERAL VIROLOGY, 1991, 72 :1215-1221

[8] A TECHNIQUE FOR RADIOLABELING DNA RESTRICTION ENDONUCLEASE FRAGMENTS TO HIGH SPECIFIC ACTIVITY [J].

FEINBERG, AP ;

VOGELSTEIN, B .

ANALYTICAL BIOCHEMISTRY, 1983, 132 (01) :6-13

[9]

GAASTRA W, 1984, METHOD MOL BIOL, V2, P67

[10] OBSERVATIONS CONCERNING THE DISCONTINUOUS DNAS OF CAULIFLOWER MOSAIC-VIRUS [J].

GUILLEY, H ;

RICHARDS, KE ;

JONARD, G .

EMBO JOURNAL, 1983, 2 (02) :277-282

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