METHOTREXATE-LOADED, PHOTOSENSITIZED ERYTHROCYTES - A PHOTO-ACTIVATABLE CARRIER/DELIVERY SYSTEM FOR USE IN CANCER-THERAPY

被引：24

作者：

FLYNN, G ^{[1
]}

MCHALE, L ^{[1
]}

MCHALE, AP ^{[1
]}

机构：

[1] UNIV ULSTER,DEPT APPL BIOL & CHEM SCI,BIOTECHNOL RES GRP,COLERAINE BT52 1SA,LONDONDERRY,NORTH IRELAND

来源：

CANCER LETTERS | 1994年 / 82卷 / 02期

关键词：

PHOTODYNAMIC THERAPY; HEMATOPORPHYRIN DERIVATIVE; CANCER; CHEMOTHERAPY; ERYTHROCYTES; ELECTROPORATION; CONTROLLED RELEASE;

D O I：

10.1016/0304-3835(94)90016-7

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

With a view towards the design of a system incorporating both the use of chemotherapeutics and photodynamic therapy for use in cancer treatment modalities, erythrocytes have been loaded with methotrexate and subsequently photosensitized by exposure to hematoporphyrin derivative. Loading of methotrexate into erythrocytes has been optimized by examining variations in electroporation conditions. Maximum loading indices observed were in the region of 64%. In order to obtain rapid pre-defined release of chemotherapeutic from the system, the erythrocytes were photosensitized. Light-dependent release of methotrexate from the system was examined. In addition, studies measuring the cytotoxic effects of light-activated release from the system using Hela cells as a target, suggested that decreases in cell viability following exposure to light resulted from the combined effects of chemotherapy and photoradiation therapy. Potential applications and advantages associated with this novel system are discussed.

引用

页码：225 / 229

页数：5

共 18 条

[1] SELECTIVE NECROSIS IN DIMETHYLHYDRAZINE-INDUCED RAT COLON TUMORS USING PHTHALOCYANINE PHOTODYNAMIC THERAPY [J].

BARR, H ;

TRALAU, CJ ;

BOULOS, PB ;

MACROBERT, AJ ;

KRASNER, N ;

PHILLIPS, D ;

BOWN, SG .

GASTROENTEROLOGY, 1990, 98 (06) :1532-1537

[2]

BIANCHI R, 1992, J CHEMOTHERAPY, V4, P290

[3]

CLINE MJ, 1980, CANCER TREATMENT, P27

[4]

DOUGHERTY TJ, 1985, METHODS PORPHYRIN PH, P301

[5] CHARACTERIZATION OF 5-FLUOROURACIL LOADED LIPOSOMES PREPARED BY REVERSE-PHASE EVAPORATION OR FREEZING-THAWING EXTRUSION METHODS - STUDY OF DRUG-RELEASE [J].

ELORZA, B ;

ELORZA, MA ;

FRUTOS, G ;

CHANTRES, JR .

BIOCHIMICA ET BIOPHYSICA ACTA, 1993, 1153 (02) :135-142

[6]

FLYNN G, 1994, IN PRESS CANCER LETT

[7] THE ULTRASTRUCTURE OF PHOTOCHEMICALLY INDUCED THROMBI WITH EMBOLIZATION IN A RAT MODEL [J].

FUTRELL, N ;

RIDDLE, JM .

STROKE, 1993, 24 (12) :1983-1992

[8] IMMUNOTARGETING CHEMOTHERAPY FOR AFP-PRODUCING PEDIATRIC LIVER-CANCER USING THE CONJUGATES OF ANTI-AFP ANTIBODY AND ANTITUMOR AGENTS [J].

HATA, Y ;

TAKADA, N ;

SASAKI, F ;

ABE, T ;

HAMADA, H ;

TAKAHASHI, H ;

UCHINO, J ;

TSUKADA, Y .

JOURNAL OF PEDIATRIC SURGERY, 1992, 27 (06) :724-727

[9]

HOLMES S, 1990, CANCER CHEMOTHERAPY, P22

[10] ERYTHROCYTE CARRIERS [J].

IHLER, GM .

PHARMACOLOGY & THERAPEUTICS, 1983, 20 (02) :151-169

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