CRYSTAL-STRUCTURE OF A MISPAIRED DODECAMER, D(CGAGAATTC(O(6)ME)GCG)2, CONTAINING A CARCINOGENIC O(6)-METHYLGUANINE

被引：15

作者：

GINELL, SL ^{[1
]}

VOJTECHOVSKY, J ^{[1
]}

GAFFNEY, B ^{[1
]}

JONES, R ^{[1
]}

BERMAN, HM ^{[1
]}

机构：

[1] RUTGERS STATE UNIV,DEPT CHEM,NEW BRUNSWICK,NJ 08903

来源：

BIOCHEMISTRY | 1994年 / 33卷 / 12期

关键词：

D O I：

10.1021/bi00178a004

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The crystal structure of the synthetic deoxydodecamer d(CGAGAATTC(O6Me)GCG)2 has been determined and refined to an R-factor of 16.9% with data up to 2.9-angstrom resolution. This sequence contains two mismatched base pairs between O6-methylguanine and adenine with the arrangement A(syn).(O6-Me)G(anti) which differs from the geometry observed in solution by NMR. The intermolecular arrangement is equivalent to the other isomorphous deoxydodecamers. However, the weakening of some significant crystal packing contacts was observed and related to the effect of stacking between the mispaired adenine and the adjacent guanine in the sequence. The structure is highly hydrated, with a total of 49 solvent molecules located. The methyl group and the mismatched base-pair geometry locally disrupt the B-DNA-type solvent network with two solvent molecules found close to the N1 and N6 of the mispaired adenine.

引用

页码：3487 / 3493

页数：7

共 39 条

[1]

BABCOCK MS, 1992, COMPUTATION BIOMOLEC, P65

[2] ONCOGENE ACTIVATION IN CHEMICAL CARCINOGENESIS [J].