DETERMINATION OF HEXOKINASE ISOENZYME-I AND ISOENZYME-II COMPOSITION BY RT-PCR - INCREASED HEXOKINASE ISOENZYME-II IN HUMAN RENAL-CELL CARCINOMA

被引：27

作者：

ADAMS, V ^{[1
]}

KEMPF, W ^{[1
]}

HASSAM, S ^{[1
]}

BRINER, J ^{[1
]}

机构：

[1] UNIV ZURICH HOSP,DEPT DERMATOL,CH-8091 ZURICH,SWITZERLAND

来源：

BIOCHEMICAL AND MOLECULAR MEDICINE | 1995年 / 54卷 / 01期

关键词：

D O I：

10.1006/bmme.1995.1008

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Hexokinase isoenzyme composition has been noted to vary in different tissues and with the developmental and metabolic status of the cell. Until now these investigations were performed either by isoenzyme electrophoresis or by column chromatography. In this report we describe an RNA-PCR method to evaluate the percentage of HK-1 and HK-2 in different rat tissues. Furthermore we applied the method to determine if a shift in isoform composition is detectable in human renal carcinomas compared to normal kidney tissue. In all of our specimens we were able to detect a shift toward HK-2 in the carcinoma specimens. We discuss a possible role for the detection of the shift in isoenzyme composition as a possible marker to discriminate between normal and malignant specimens. (C) 1995 Academic Press, Inc.

引用

页码：53 / 58

页数：6

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