ENZYMATIC METHOD FOR DETERMINATION OF BRANCHED-CHAIN AMINO-ACID AMINOTRANSFERASE ACTIVITY

被引：9

作者：

SCHADEWALDT, P

HUMMEL, W

WENDEL, U

ADELMEYER, F

机构：

[1] FORSCHUNGSZENTRUM JULICH, DIABET FORSCHUNGSINST, JULICH, GERMANY

[2] FORSCHUNGSZENTRUM JULICH, INST ENZYMTECHNOL, JULICH, GERMANY

[3] UNIV DUSSELDORF, KINDERKLIN, D-40225 DUSSELDORF, GERMANY

来源：

ANALYTICAL BIOCHEMISTRY | 1995年 / 230卷 / 02期

关键词：

D O I：

10.1006/abio.1995.1464

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A spectrophotometric assay for the determination of branched-chain L-amino acid aminotransferase activity is described. It is based on the transamination of L-leucine in the presence of 2-oxoglutarate yielding 4-methyl-2-oxopentanoate. The rate of formation of the branched-chain 2-oxo acid is specifically monitored in a coupled enzymatic reaction using NAD(+)-dependent D-2-hydroxyisocaproate dehydrogenase from Lactobacillus casei ssp. pseudoplantarum as coupling enzyme by measuring the decrease in NADH absorbance at 334 nm. Optimized assay conditions are provided as evaluated for the (iso)enzyme from rat heart. (C) 1995 Academic Press, Inc.

引用

页码：199 / 204

页数：6

共 39 条

[1]

AIRAS RK, 1989, BIOCHIM BIOPHYS ACTA, V992, P397

[2] ONE-STEP AND 2-STEP FLUOROMETRIC ASSAY-METHODS FOR GENERAL AMINOTRANSFERASES USING GLUTAMATE-DEHYDROGENASE [J].