BASIC FIBROBLAST GROWTH-FACTOR SENSE (FGF) AND ANTISENSE (GFG) RNA TRANSCRIPTS ARE EXPRESSED IN UNFERTILIZED HUMAN OOCYTES AND IN DIFFERENTIATED ADULT TISSUES

Basic fibroblast growth factor (bFGF) is a highly conserved and ubiquitously distributed mitogen. In amphibian oocytes bFGF mRNA is regulated post-transcriptionally by interaction with an antisense RNA transcript. We used reverse transcription-polymerase chain reaction (RT-PCR) and Northern hybridization to determine the presence of bFGF and its antisense RNA (gfg) in unfertilized human oocytes and postnatal differentiated tissues. BFGF and gfg transcripts were co-expressed in many tissues, with bFGF transcripts (7, 3.7 and 1.8 kb) being more abundant than the gfg transcript (1.5 kb) in 8 of 16 tissues examined. Sense and antisense expression was approximately equal in kidney and colon, while in heart, liver, skeletal muscle and testis gfg transcripts predominated. RT-PCR demonstrated the presence of bFGF and gfg transcripts in unfertilized oocytes where the antisense transcript was present in excess of the sense transcript. These findings suggest a role for gfg in regulation of bFGF expression. (C) 1994 Academic Press, Inc.