ION-EXCHANGE CHROMATOGRAPHY OF SACCHARIDES - AN IMPROVED SYSTEM UTILIZING BORIC ACID/2,3-BUTANEDIOL BUFFERS

被引：28

作者：

WALBORG, EF

RAY, DB

OHRBERG, LE

机构：

[1] The University of Texas M. D. Anderson Hospital, Tumor Institute at Houston, Department of Biochemistry, Houston

来源：

ANALYTICAL BIOCHEMISTRY | 1969年 / 29卷 / 03期

关键词：

D O I：

10.1016/0003-2697(69)90328-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An improved ion-exchange column chromatographic system for the separation and quantitation of neutral mono-, di-, and trisaccharides is described. A two-buffer, stepwise elution system, utilizing a 0.6 × 85 cm column of Dowex 1-X4, eluted with boric acid/2,3-butanediol buffers at a flow rate of 70 ml/cm2/hr and operated at elevated temperatures has been developed for the resolution of complex mixtures of saccharides. Analysis of the column effluents with the aniline/acetic acid/orthophosphoric acid reagent permits quantitation of the saccharides with a precision of approximately ± 5%. © 1969.

引用

页码：433 / &

共 12 条

[1] FRAHN JL, 1956, CHEM IND-LONDON, P578
[2] GREEN JG, 1966, 21 NATL CANC I MON, P447
[3] CHROMATOGRAPHIC ANALYSIS OF SUGARS AS COMPLEX BORATE IONS
HALLEN, A
[J]. ACTA CHEMICA SCANDINAVICA, 1960, 14 (10): : 2249 - 2250
[4] JOLLEY RL, 1968, CLIN CHEM, V14, P538
[5] RAPID QUANTITATIVE ANION-EXCHANGE CHROMATOGRAPHY OF CARBOHYDRATES
KESLER, RB
[J]. ANALYTICAL CHEMISTRY, 1967, 39 (12) : 1416 - &
[6] THE SEPARATION OF SUGARS BY ION EXCHANGE
KHYM, JX
ZILL, LP
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1952, 74 (08) : 2090 - 2094
[7] THE SEPARATION OF MONOSACCHARIDES BY ION EXCHANGE
KHYM, JX
ZILL, LP
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1951, 73 (05) : 2399 - 2400
[8] CHROMATOGRAPHY OF AMINO ACIDS ON SULFONATED POLYSTYRENE RESINS - AN IMPROVED SYSTEM
MOORE, S
SPACKMAN, DH
STEIN, WH
[J]. ANALYTICAL CHEMISTRY, 1958, 30 (07) : 1185 - 1190
[9] COLUMN CHROMATOGRAPHY OF NEUTRAL SUGARS - OPERATING CHARACTERISTICS AND PERFORMANCE OF A NEWLY AVAILABLE ANION-EXCHANGE RESIN
OHMS, JI
ZEC, J
BENSON, JV
PATTERSO.JA
[J]. ANALYTICAL BIOCHEMISTRY, 1967, 20 (01) : 51 - &
[10] SEPARATION AND QUANTITATION OF SACCHARIDES BY ION-EXCHANGE CHROMATOGRAPHY UTILIZING BORIC ACID/GLYCEROL BUFFERS
WALBORG, EF
LANTZ, RS
[J]. ANALYTICAL BIOCHEMISTRY, 1968, 22 (01) : 123 - &

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